{"created":"2023-07-27T07:00:31.968637+00:00","id":60496,"links":{},"metadata":{"_buckets":{"deposit":"e928b7ee-60bd-4c3c-a4ed-2df6138b5ff5"},"_deposit":{"created_by":18,"id":"60496","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60496"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060496","sets":["2812:2813:2821"]},"author_link":["572"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-07-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"2010-04-01 – 2013-03-31","bibliographic_titles":[{"bibliographic_title":"平成24(2012)年度 科学研究費補助金 若手研究(B) 研究課題概要"},{"bibliographic_title":"2012 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本年度は,肺癌におけるEGFR(epidermal growth factor receptor)蛋白の過剰発現,EGFR遺伝子増幅,EGFR遺伝子変異,PDGF(platelet-derived growth factor)の発現について検索した.\n1.肺癌におけるEGFR蛋白過剰発現の免疫染色による検索\n159例の肺癌のホルマリン固定・パラフィン包埋組織で抗EGFR抗体を用いて免疫染色を行ったところ,EGFRの過剰発現は61例(38%)の症例にみられた.\n2.FISH(fluorescence in situ hybridization)法によるEGFR遺伝子増幅の検索\n上記EGFR過剰発現を示す61症例に対してEGFR(7p12)と染色体7セントロメアを認識する2色プローブを用いた2色FISH法を行ったところ,11例でEGFR遺伝子増幅,11例で7番染色体polysomyがみられた.\n3.ダイレクトシークエンス法によるEGFR遺伝子変異の検索\n159例の肺癌凍結標本から抽出したDNAを用いて,EGFR遺伝子のexon18-21のダイレクトシークエンス法を行ったところ,60例(38%)においてEGFR遺伝子変異がみられた.EGFR遺伝子コピー数の増加を示さない群(137例)の37%,EGFR遺伝子増幅を示す群(11例)の55%,polysomyを有する群(11例)の33%の症例で変異がみられた.\n4.肺癌におけるPDGF蛋白発現の免疫染色による検索\nPDGFの発現は全体の90%の症例でみられ,さらに24%の症例において中等度以上の染色強度がみられた.\n上記1-4.で得られた結果の一部は,他の実験結果とともに学術雑誌上および学会において報告した(下記「雑誌論文」と「学会発表」の項目を参照).","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:22790340, 研究期間(年度):2010-04-01 – 2013-03-31","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「肺癌に対する分子標的薬剤併用療法適応拡大の検討」課題番号22790340\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22790340/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学系","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00066743","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40334859"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40334859","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22790340/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-22790340/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-08"}],"displaytype":"detail","filename":"ME-PR-SUZUKI-S-kaken 2019-2p.pdf","filesize":[{"value":"144.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-SUZUKI-S-kaken 2019-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60496/files/ME-PR-SUZUKI-S-kaken 2019-2p.pdf"},"version_id":"33c6c5c2-7186-470f-8c32-1cc692aaae17"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"肺癌に対する分子標的薬剤併用療法適応拡大の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"肺癌に対する分子標的薬剤併用療法適応拡大の検討"}]},"item_type_id":"9","owner":"18","path":["2821"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-07-08"},"publish_date":"2022-07-08","publish_status":"0","recid":"60496","relation_version_is_last":true,"title":["肺癌に対する分子標的薬剤併用療法適応拡大の検討"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:40:14.779993+00:00"}