{"created":"2023-07-27T07:00:46.902629+00:00","id":60834,"links":{},"metadata":{"_buckets":{"deposit":"a1d6e2a3-4872-4936-b3e1-0256af46ab5e"},"_deposit":{"created_by":18,"id":"60834","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60834"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060834","sets":["1132:1133:1135"]},"author_link":["106973","106971","106948","17798","106954","106970","106955"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23","bibliographicPageStart":"5947","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"古川, 敦"}],"nameIdentifiers":[{"nameIdentifier":"106955","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30727699","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30727699"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The human leucocyte antigen (HLA)-G, which consists of seven splice variants, is a tolerogenic immune checkpoint molecule. It plays an important role in the protection of the fetus from the maternal immune response by binding to inhibitory receptors, including leukocyte Ig-like receptors (LILRs). Recent studies have also revealed that HLA-G is involved in the progression of cancer cells and the protection from autoimmune diseases. In contrast to its well characterized isoform, HLA-G1, the binding activities of other major HLA-G isoforms, such as HLA-G2, toward available anti-HLA-G antibodies are only partially understood. Here, we investigate the binding specificities of anti-HLA-G antibodies by using surface plasmon resonance. MEM-G9 and G233 showed strong affinities to HLA-G1, with a nM range for their dissociation constants, but did not show affinities to HLA-G2. The disulfide-linker HLA-G1 dimer further exhibited significant avidity effects. On the other hand, 4H84 and MEM-G1, which can be used for the Western blotting of HLA-G isoforms, can bind to native HLA-G2, while MEM-G9 and G233 cannot. These results reveal that HLA-G2 has a partially intrinsically disordered structure. Furthermore, MEM-G1, but not 4H84, competes with the LILRB2 binding of HLA-G2. These results provide novel insight into the functional characterization of HLA-G isoforms and their detection systems. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067079","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI AG"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms20235947","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.mdpi.com/1422-0067/20/23/5947"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/1422-0067/20/23/5947","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.mdpi.com/journal/ijms"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/journal/ijms","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.mdpi.com/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.mdpi.com/","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1661-6596","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Furukawa, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"106955","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30727699","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30727699"}]},{"creatorNames":[{"creatorName":"Meguro, Manami"}],"nameIdentifiers":[{"nameIdentifier":"106970","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamazaki, Rika"}],"nameIdentifiers":[{"nameIdentifier":"106971","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"17798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Ami"}],"nameIdentifiers":[{"nameIdentifier":"106973","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuroki, Kimiko"}],"nameIdentifiers":[{"nameIdentifier":"106948","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maenaka, Katsumi"}],"nameIdentifiers":[{"nameIdentifier":"106954","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-12"}],"displaytype":"detail","filename":"PH-PR-FURUKAWA-A-20-05947.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-FURUKAWA-A-20-05947.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60834/files/PH-PR-FURUKAWA-A-20-05947.pdf"},"version_id":"065d1c76-6eaa-4f0e-8697-7e4e2f9322cf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of the reactivity and receptor competition of HLA-G isoforms toward available antibodies: Implications of structural characteristics of HLA-G isoforms","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of the reactivity and receptor competition of HLA-G isoforms toward available antibodies: Implications of structural characteristics of HLA-G isoforms"}]},"item_type_id":"4","owner":"18","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-12"},"publish_date":"2022-09-12","publish_status":"0","recid":"60834","relation_version_is_last":true,"title":["Evaluation of the reactivity and receptor competition of HLA-G isoforms toward available antibodies: Implications of structural characteristics of HLA-G isoforms"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-06-20T06:57:07.940691+00:00"}