{"created":"2023-07-27T07:00:47.037513+00:00","id":60837,"links":{},"metadata":{"_buckets":{"deposit":"04eb2172-bde2-437a-a727-b51ab4d7cdda"},"_deposit":{"created_by":18,"id":"60837","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60837"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060837","sets":["1132:1133:1135"]},"author_link":["99989","106943","107001","106969","106955","107000"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"e1263","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"PLoS Neglected Tropical Diseases"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"古川, 敦"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mitochondrion-related organelles, mitosomes and hydrogenosomes, are found in a phylogenetically broad range of organisms. Their components and functions are highly diverse. We have previously shown that mitosomes of the anaerobic/microaerophilic intestinal protozoan parasite Entamoeba histolytica have uniquely evolved and compartmentalized a sulfate activation pathway. Although this confined metabolic pathway is the major function in E. histolytica mitosomes, their physiological role remains unknown. In this study, we examined the phenotypes of the parasites in which genes involved in the mitosome functions were suppressed by gene silencing, and showed that sulfate activation in mitosomes is important for sulfolipid synthesis and cell proliferation. We also demonstrated that both Cpn60 and unusual mitochondrial ADP/ATP transporter (mitochondria carrier family, MCF) are important for the mitosome functions. Immunoelectron microscopy demonstrated that the enzymes involved in sulfate activation, Cpn60, and mitochondrial carrier family were differentially distributed within the electron dense, double membrane-bounded organelles. The importance and topology of the components in E. histolytica mitosomes reinforce the notion that they are not \"rudimentary\" or \"residual\" mitochondria, but represent a uniquely evolved crucial organelle in E. histolytica. © 2011 Mi-ichi et al.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067082","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pntd.0001263","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001263"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001263","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.plosNTDS.org/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plosNTDS.org/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.plos.org/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.plos.org/","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 Mi-ichi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."},{"subitem_rights":"This is an Open Access article under the CC BY license."}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1935-2727","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1935-2735","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mi-ichi, Fumika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Makiuchi, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furukawa, Atsushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sato, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nozaki, Tomoyoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-12"}],"displaytype":"detail","filename":"PH-PR-FURUKAWA-A-5-1263.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-FURUKAWA-A-5-1263.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60837/files/PH-PR-FURUKAWA-A-5-1263.pdf"},"version_id":"b6881fd8-0b0d-4e2a-9ccc-6efb33f1910d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sulfate activation in mitosomes plays an important role in the proliferation of entamoeba histolytica","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sulfate activation in mitosomes plays an important role in the proliferation of entamoeba histolytica"}]},"item_type_id":"4","owner":"18","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-09-12"},"publish_date":"2022-09-12","publish_status":"0","recid":"60837","relation_version_is_last":true,"title":["Sulfate activation in mitosomes plays an important role in the proliferation of entamoeba histolytica"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:29:17.064098+00:00"}