{"created":"2023-07-27T07:00:50.973015+00:00","id":60929,"links":{},"metadata":{"_buckets":{"deposit":"d6192ce8-0804-4185-8ae8-e7485b53a4e0"},"_deposit":{"created_by":18,"id":"60929","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60929"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060929","sets":["2812:2813:2842"]},"author_link":["23597","31327"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1989 – 1991","bibliographic_titles":[{"bibliographic_title":"平成3(1991)年度 科学研究費補助金 一般研究(B) 研究成果報告書概要"},{"bibliographic_title":"1991 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamamoto, Chosaburo"}],"nameIdentifiers":[{"nameIdentifier":"23597","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50008231","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50008231"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1.海馬体の苔状線維終末からの伝達物質放出について確実な結論を得るには、素量解析法によらなければならない。そのために私達はテンジクネズミの海馬体薄切片において単一苔状線維を刺激し、それによって単シナプス性にCA3野ニュ-ロンに発生する興奮性シナプス後電位(EPSP)を記録する方法を確立した。このようにして記録される単位EPSPの振幅分布がパスカル分布に合うことが発見された。この発見に基いて、実験から得たEPSPの振幅分布に最もよく適合するパスカル分布のパラメタを最尤法によって定める素量解析法を確立した。\n2.アセチルコリンやノルアドレナリンを外液中に加えて組繊に適用したが、単位EPSPの振幅に再現性のある変化を起こすことはできなかった。\n3.ガンマアミノ酪酸(GABA)のB受容体作動薬であるバクロフェンは、単位EPSPを可逆的に抑制した。このバクロフェンの作用を素量解析法によって吟味したところ、平均素量放出数はバクロフェン1μMにおいて14%、10μMにおいて59%減少することが見出された。平均素量振幅はバクロフェン1μMにおいて18%,10μMにおいて21%減少した。この結果は、苔状線維終末のGABAーB受容体が伝達物質の放出を抑制的に修飾することを示している。\n4.アデノシンは10μMの濃度で、単位EPSPの振幅を対照の57%に低下させた。このとき、平均素量放出数は51%まで減少した。平均素量振幅はほとんど変化しなかった。この結果から、アデノシンは伝達物質と共に放出され、伝達物質の放出に抑制的修飾をおよぼしているという結論を得た。","subitem_description_type":"Abstract"},{"subitem_description":"Information on release of neurotransmitter from central synaptic boutons can be obtained only by a rigorous quantal analysis. To establish a quantal analysis method in hippocampus, we developed a technique to activate a single mossy fiber, axon of granule cell, and record excitatory postsynaptic potentials (EPSPS) from a CA3 neuron innervated by the particular granule cell in thin transverse sections of the guinea pig hippocampus. The amplitude distribution of the unitary EPSPs recorded by this method was described by the Pascal statistics. The values of mean quantal content (m) and mean quantal amplitude (q) could be estimated by the maximum likelihood method.\nAcetylcholine and-norepinephrine administered to the perfusing solution failed to induce reproducible changes in the unitary EPSPS.\nBaclofen, an agonist of the GABA-B receptor, suppressed the unitary EPSPs reversibly. This suppression was accompanied by decreases in m (14% at 1 muM and 59% at 10 muM baclofen). The q value was decreased by 18% at 1 muM and by 21% at 10muM. These results indicate that the GABA-B receptor at the mossy fiber terminal modulates release of transmitter therefrom.\nAdenosine suppressed the unitary EPSPs to 57% at 10 muM. Them value was reduced to 51% under the action of adenosine, while no significant changes were detected in q. From these results, it is concluded that the adenosine terminal exerts inhibitory modulating action on release of transmitter.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:01480121, 研究期間(年度):1989 – 1991","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「中枢シナプスにおける伝達物質放出の修飾機構ー素量解析による研究」課題番号01480121\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480121/014801211991kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067173","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=50008231"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=50008231","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01480121/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01480121/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480121/014801211991kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480121/014801211991kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山本, 長三郎"}],"nameIdentifiers":[{"nameIdentifier":"31327","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50008231","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50008231"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-20"}],"displaytype":"detail","filename":"ME-PR-YAMAMOTO-C-kaken 1993-2p.pdf","filesize":[{"value":"87.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-YAMAMOTO-C-kaken 1993-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60929/files/ME-PR-YAMAMOTO-C-kaken 1993-2p.pdf"},"version_id":"1f317bf3-5385-402c-999f-cb9987ecb0dc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"中枢シナプスにおける伝達物質放出の修飾機構: 素量解析による研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"中枢シナプスにおける伝達物質放出の修飾機構: 素量解析による研究"},{"subitem_title":"Modulating Mechanisms of Neurotransmitter Release in Central Synapses --- Investigation by a Quantal Analysis","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2842"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-20"},"publish_date":"2022-10-20","publish_status":"0","recid":"60929","relation_version_is_last":true,"title":["中枢シナプスにおける伝達物質放出の修飾機構: 素量解析による研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:47:22.667732+00:00"}