{"created":"2023-07-27T07:00:51.018844+00:00","id":60930,"links":{},"metadata":{"_buckets":{"deposit":"aedc2150-aa83-47b1-92b7-f26c02618bec"},"_deposit":{"created_by":18,"id":"60930","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60930"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060930","sets":["2812:2813:2843"]},"author_link":["26399","20437"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-08-11","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1989 – 1990","bibliographic_titles":[{"bibliographic_title":"平成2(1990)年度 科学研究費補助金 一般研究(B) 研究成果報告書概要"},{"bibliographic_title":"1990 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト末梢血細胞のうち、種々の刺激によりILー1、ILー6を産生する主たる細胞は単球であることを免疫疫組織染色、in situ hybridization法により確認し、臍帯血、成人末梢血を全血のまま刺激する方法でこれらのサイトカイン産生能を検討した。生下時体重1,800gm以上の児ではこれらのサイトカイン産生能は既に成人のレベルに達していたが、存胎30週未満、生下時体重1,500gm以下の極小未熟児では種々の刺激によるILー6の産生が不良なことから、単球のモノカイン産生態は在胎30週前後に成熟に達するものと推察される。また、骨髄移植後のIL6産生能も、かなり早い時期に獲得されるようである\nヒト末梢血T細胞におけるCD45抗原群の発現状態を見ると、臍帯血T細胞の殆どはCD45ROー,CD45RA+の形質をもつナイ-ブT細胞からなるが、年令の進むにつれCD45Ro+(メモリ-)T細胞の比率が増す。ウイルス感染症では急性期にCD45RO+T細胞の著増がみられ、回復期にいたり次第2減少する現象がみられ、ヒト末梢血のCD45RO+T細胞の比率は、その個体が生後に受けたな抗原刺激の総和を反映するものかもしれない。\n臍帯血のCD4+T細胞は殆どがCD45RA+,CD45RO-(ナイ-ブ)T細胞からなり、IL-4,IL-5,IFN-γ産生態を欠き、B細胞の免疫グロブリン産生をヘルプし得ないが、数日間のin vitro PHA刺激によりCD45RA+、RO-からCD45RA-,RO+への形質変換がおこり、これに伴ってIL-4,IL-5,IFN-γなどのサイトカイン産生態、ヘルパ-能が出現することが確かめられた。\n単離したナイ-ブT細胞を抗CD2抗体で刺激しても増殖反応はみられないが、メモリ-T細胞は旺盛な増殖をしめす。メモリ-T細胞は抗CD2抗体刺激により自らが微量のIL-6を産生し、オ-トクリン機構を介して増殖し得るが、ナイ-ブT細胞はIL-6産生能を欠くため、IL-6ソ-スとしての単球の共存なしには増殖し得ないことが明らかにされた。","subitem_description_type":"Abstract"},{"subitem_description":"Several kinds of cytokines were identified in blood plasma when heparinized whole blood was stimulated directly with various stimuli, such as bacteria, bacterial products, and lectins. Immunohistologic and in situ hybridization studies confirmed that IL-1, IL-6, and EFN-gamma were produced almost exclusively by stimulated monocytes in this whole blood system. IL-6 producing ability of cord blood from neonates with over 1,800 gm of birth weight was comparable to adult controls, while very low birth weight infants born at less than 30 weeks of gestation weighing less than 1,500 gm showed poor ability to produce IL-6.\nIt has been claimed that isoform expression patterns of CD45 antigen family denote mutually exclusive two T cell subsets : \"naive\" T cells expressing CD45RA^+, RO^- and \"memory T cells with CD45RO^-, RO^+ phenotype. Almost all T cells in cord blood expressed naive phenotype. Relative proportions of circulating memory T cells increased with advancing age and reached adult levels around 12 years of age.\nBy using reverse transcriptase/PCR technology, it was confirmed that CD4^+CD45RO^- naive T cells could not express mRNAs for IL-4, IL-5, and IFN-gamma on stimulation. CD4^+, CD45RO^+ memory T cells had the ability to produce these lymphokines. In addition, CD4^+ naive T cells as well as whole cord CD4^+ T cells exerted no significant help for B cell differentiation, while CD4^+ memory T cells acted as an efficient helper in PWM- stimulated coculture system. In vitro stimulation of CD4^+ naive T cells and whole cord CD4^+ T cells with PHA in the presence of exogenous IL-2 caused phenotypic conversion from CD45RO^- to CD45RO^+. Following phenotype conversion, originally naive CD4^+ T cells acquired some memory T cellーlike function.\nFACS-purified CD4^+ naive T cells could not respond to a combination of anti-CD2 antigens in the absence of monocytes, whereas CD4^+ memory T cells showed vigorous proliferation. CD4^+ memory T cells, but not CD4^+ naive T cells, could produce IL-6 in response to anti-CD2. Whether IL-6 was produced or not seems to be crucial for anti-CD-2mediated cellular proliferation.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:01480257, 研究期間(年度):1989 – 1990","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「新生児および骨髄移植患者のサイトカイン産生態と感染象の特異性に関する研究」課題番号01480257\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480257/014802571990kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067174","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10019888"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10019888","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01480257/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01480257/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480257/014802571990kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-01480257/014802571990kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-21"}],"displaytype":"detail","filename":"ME-PR-TANIGUCHI-N-kaken 1993-2p.pdf","filesize":[{"value":"89.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TANIGUCHI-N-kaken 1993-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/60930/files/ME-PR-TANIGUCHI-N-kaken 1993-2p.pdf"},"version_id":"666cf0a7-209f-4c0c-a0ec-ac5c1b1d04ed"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"新生児および骨髄移植患者のサイトカイン産生態と感染象の特異性に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新生児および骨髄移植患者のサイトカイン産生態と感染象の特異性に関する研究"},{"subitem_title":"Cytokine Status and Susceptibility to Specified Pathogens in Human Neonates and in Recipients of Marrow Grafts","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2843"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-21"},"publish_date":"2022-10-21","publish_status":"0","recid":"60930","relation_version_is_last":true,"title":["新生児および骨髄移植患者のサイトカイン産生態と感染象の特異性に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:08:41.263258+00:00"}