{"created":"2023-07-27T07:00:53.295957+00:00","id":60980,"links":{},"metadata":{"_buckets":{"deposit":"267ab49c-8413-4548-877c-1ad1284d3739"},"_deposit":{"created_by":18,"id":"60980","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"60980"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00060980","sets":["2812:2813:2844"]},"author_link":["36","68266"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1989","bibliographic_titles":[{"bibliographic_title":"平成1(1989)年度 科学研究費補助金 重点領域研究 研究課題概要"},{"bibliographic_title":"1989 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Higashida, Haruhiro"}],"nameIdentifiers":[{"nameIdentifier":"68266","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30093066","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30093066"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1.アセチ-ルコリンを、伝達物質とするニュ-ロブラスト-マ由来NG108-15細胞を培養した。\n2.NG108-15細胞を、マウス胎児後肢横紋筋細胞の上に培養(co-culture)した。\n3.NG108-15細胞、筋細胞両方に、ガラス微小電極を刺入し膜電位の測定を行った。\nシナプスス反応(M.E.P.P.S)を記録し、NG108-15細胞がアセチ-ルコリンを放出し、筋細胞がそのアセチ-ルコリンに反応している事を確認した。\n4.NG108ー15細胞に、さらにもう1本、IP_3を充めたガラス管を刺入し、電気泳動的に細胞内にIP_3を注入し、その際、シナプス反応が増加することを確認した。\n5.IP_3によるシナプス反応の増加(プレシナブスファシリテ-ション)が、細胞外液中のCaによらないものである事を確認するため、IP_3注入時のシナプス反応促進を、Ca^<2+>フリ-条件下やCaブロッカ-であるニフェジピンやBa^<2+>存在下で測定した。\n6.以上、IP_3注入時に生じるファシリテ-ションはプレシナプス膜の過分極でもなく、また細胞外から流入するCaでもないことを確認した。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:01641512, 研究期間(年度):1989","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「イノシト-ルリン脂質代謝産物によるK+チャンネルの制御機構」課題番号01641512\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01641512/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067224","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=30093066"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=30093066","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01641512/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-01641512/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"東田, 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