{"created":"2023-07-27T07:00:55.664877+00:00","id":61032,"links":{},"metadata":{"_buckets":{"deposit":"121bdea4-c93a-427c-8cae-cb475448503d"},"_deposit":{"created_by":18,"id":"61032","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61032"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061032","sets":["1132:1133:1135"]},"author_link":["107553","93032","107551","107556","97949","107552","107548","107559","107549","107558","78857","107547","78856","107554","24438"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-09-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"2370","bibliographicPageStart":"2359","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Emerging Microbes & Infections"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"坂本, 明彦"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"水野, 哲志"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"伊從, 光洋"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"吉田, 栄人"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Viral vectors are a potent vaccine platform for inducing humoral and T-cell immune responses. Among the various viral vectors, replication-competent ones are less commonly used for coronavirus disease 2019 (COVID-19) vaccine development compared with replication-deficient ones. Here, we show the availability of a smallpox vaccine LC16m8Δ (m8Δ) as a replication-competent viral vector for a COVID-19 vaccine. M8Δ is a genetically stable variant of the licensed and highly effective Japanese smallpox vaccine LC16m8. Here, we generated two m8Δ recombinants: one harbouring a gene cassette encoding the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein, named m8Δ-SARS2(P7.5-S)-HA; and one encoding the S protein with a highly polybasic motif at the S1/S2 cleavage site, named m8Δ-SARS2(P7.5-SHN)-HA. M8Δ-SARS2(P7.5-S)-HA induced S-specific antibodies in mice that persisted for at least six weeks after a homologous boost immunization. All eight analysed serum samples displayed neutralizing activity against an S-pseudotyped virus at a level similar to that of serum samples from patients with COVID-19, and more than half (5/8) also had neutralizing activity against the Delta/B.1.617.2 variant of concern. Importantly, most serum samples also neutralized the infectious SARS-CoV-2 Wuhan and Delta/B.1.617.2 strains. In contrast, immunization with m8Δ-SARS2(P7.5-SHN)-HA elicited significantly lower antibody titres, and the induced antibodies had less neutralizing activity. Regarding T-cell immunity, both m8Δ recombinants elicited S-specific multifunctional CD8+ and CD4+ T-cell responses even after just a primary immunization. Thus, m8Δ provides an alternative method for developing a novel COVID-19 vaccine.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067276","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor and Francis"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1080/22221751.2022.2122580","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2122580"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2122580","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.tandfonline.com/journals/TEMI"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.tandfonline.com/journals/TEMI","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.tandf.co.uk/journals/default.asp"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.tandf.co.uk/journals/default.asp","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd"},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2222-1751","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakamoto, Akihiko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Osawa, Hiroaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashimoto, Hinata"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mizuno, Tetsushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hasyim, Ammar A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Yu-ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okahashi, Yuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogawa, Ryohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iyori, Mitsuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Shida, Hisatoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Shigeto"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-17"}],"displaytype":"detail","filename":"PH-PR-SAKAMOTO-A-11-2359.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-SAKAMOTO-A-11-2359.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61032/files/PH-PR-SAKAMOTO-A-11-2359.pdf"},"version_id":"5daf8082-b108-42b3-8b91-e2fd32ceb0f7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine"}]},"item_type_id":"4","owner":"18","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-17"},"publish_date":"2022-10-17","publish_status":"0","recid":"61032","relation_version_is_last":true,"title":["A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:11:08.428080+00:00"}