{"created":"2023-07-27T07:00:56.624118+00:00","id":61055,"links":{},"metadata":{"_buckets":{"deposit":"01b8aab0-d7ed-4f7b-9939-8a1e865e6a2d"},"_deposit":{"created_by":18,"id":"61055","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61055"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061055","sets":["2812:2813:2843"]},"author_link":["36","68266"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-21","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1990","bibliographic_titles":[{"bibliographic_title":"平成2(1990)年度 科学研究費補助金 重点領域研究 研究課題概要"},{"bibliographic_title":"1990 Research Project Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Higashida, Haruhiro"}],"nameIdentifiers":[{"nameIdentifier":"68266","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30093066","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30093066"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"我々はすでに、バッチクランプ電流測定法により、プラジキニン(BK)が、細胞内セカンドメッセンジャ-を使ってチャンネル活動を制御していることを証明した。40pSの大きさを持つCa^<2+>依存性K^+チャンネルが細胞内に注入したイノシト-ルリン酸(IP_3)に反応することを確かめた。\nそこで本年度は、IP_3が、伝達物質をトリガ-するか否かについて検討した。アセチ-ルコリンを、伝達物質とするニュ-ロブラスト-マ由来NG108ー15細胞を培養し、横紋筋と重層培養を行った。それぞれの細胞から膜電位を記録した。NG108ー15細胞にIP_3を注入し、筋細胞でシナプス電位の増加を観察した。一方、Furaー2を用いた、細胞内Ca動態を、測定し受容体依存性ファシリテ-ションにIP_3依存的Ca^<2+>が関与することを実証した。\n以上のことは、受容体刺激により生じる伝達物質放出促進(プレシナプスファシリテ-ション)が、細胞内Ca濃度上昇により、膜電位にしないことを示す。さらに、IP_3依存性の放出に関しては、細胞外Caの流入にはよらないことがわかった。","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:02241205, 研究期間(年度):1990","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「イノシト-ル三リン酸(IP_3)のプレシナプス(伝達物質放出)作用」課題番号02241205\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02241205/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067299","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=30093066"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=30093066","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02241205/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02241205/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"東田, 陽博"}],"nameIdentifiers":[{"nameIdentifier":"36","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30093066","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30093066"},{"nameIdentifier":"30093066","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30093066"},{"nameIdentifier":"30093066","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030093066"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-14"}],"displaytype":"detail","filename":"ME-PR-HIGASHIDA-H-kaken 2016-2p.pdf","filesize":[{"value":"122.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-HIGASHIDA-H-kaken 2016-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61055/files/ME-PR-HIGASHIDA-H-kaken 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