{"created":"2023-07-27T07:00:57.642934+00:00","id":61076,"links":{},"metadata":{"_buckets":{"deposit":"38a352e0-395b-4c7b-80aa-750e59560b18"},"_deposit":{"created_by":18,"id":"61076","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61076"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061076","sets":["2812:2813:2841"]},"author_link":["105611"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1990 – 1992","bibliographic_titles":[{"bibliographic_title":"平成4(1992)年度 科学研究費補助金 一般研究(C) 研究成果報告書概要"},{"bibliographic_title":"1992 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"「コリン作動性ニューロン固有遺伝子の解析」という課題で3年に亘り、神経系興奮伝達機構の遺伝子解析を行った。初年度に、センチュウC.elegansを用いコリン作動性ニューロン、特にシナプス前終末に働く遺伝子変異体を分離し、遺伝子座も決定した。続いて、その中で中心的役割を果すunc-18遺伝子をクローニングし、塩基配列を決定し、その遺伝子がシナプス小胞の放出に関与していることを明らかにした。続いて、この遺伝子のcDNAを発現ベクターに組み込み大量の遺伝子産物を得、抗体を作製した。抗体を利用して組織化学によって、この遺伝子が運動ニューロンのシナプス前節に局在していることを明らかにした。更に、C.elegansからシナプス分離法を確立し、unc-18遺伝子産物との関連性を検討している。unc-18遺伝子変異と似た表現型を示す、従って類似機能に働くと考えられる、unc-11,unc-13,unc-17,unc-41,unc-63及びunc-64も同定している。現在これら遺伝子相互の関連性を調べている。またunc-41遺伝子のクローニングにも成功し、塩基配列の決定、遺伝子産物の性状を調べている。","subitem_description_type":"Abstract"},{"subitem_description":"Three years ago, we started our project entitled, \"Genetic analysis of cholinergic neurons\". We established the method for the screening of mutants showing abnormalities in cholinergic neurons. With this method we isolated several mutants and identified genes contributing to the mutants. Of these genes, we focused our work on genes functioning at the presynaptic terminals.\nSeven such genes (unc-11, unc-13, unc-17, unc-18, unc-41, unc-63 and unc-64) were identified. We found the genes function in the regulatory pathway controlling acetylcholine levels. To investigate further these genes at the molecular level, we cloned the unc-18 gene, one of the seven genes. DNAs of the genomic and the cDNA clone were sequenced and the deduced amino acid sequence of the cDNA was determined. Comparison of the predicted amino acid sequence revealed has a role in the release of the neurotransmitters.\nWe cloned the cDNA into the expression vector, which permit to produce the unc-18 gene product and made antisera against the gene product. With the antisera we stained animals and found the gene product is specifically localized at motor neurons. These findings indicate that UNC-18 has a role in the axonal transport and influences acetylcholine flow in motor neurons.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:02808047, 研究期間(年度):1990 – 1992","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「コリン作動性ニューロン固有遺伝子の解析」課題番号02808047\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02808047/028080471992kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067320","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=40019617"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=40019617","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02808047/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02808047/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02808047/028080471992kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02808047/028080471992kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-27"}],"displaytype":"detail","filename":"ME-PR-HOSONO-R-kaken 1994-2p.pdf","filesize":[{"value":"94.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-HOSONO-R-kaken 1994-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61076/files/ME-PR-HOSONO-R-kaken 1994-2p.pdf"},"version_id":"87e02973-9834-45be-bc30-49884158dd64"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"コリン作動性ニューロン固有遺伝子の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"コリン作動性ニューロン固有遺伝子の解析"},{"subitem_title":"Genetic analysis of cholinergic neurons","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2841"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-27"},"publish_date":"2022-10-27","publish_status":"0","recid":"61076","relation_version_is_last":true,"title":["コリン作動性ニューロン固有遺伝子の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:02:11.262331+00:00"}