{"created":"2023-07-27T07:00:58.017482+00:00","id":61084,"links":{},"metadata":{"_buckets":{"deposit":"cce278d4-bb15-4cd0-805b-7365d50a5012"},"_deposit":{"created_by":18,"id":"61084","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61084"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061084","sets":["2812:2813:2842"]},"author_link":["47652","21708"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1990 – 1991","bibliographic_titles":[{"bibliographic_title":"平成3(1991)年度 科学研究費補助金 一般研究(C) 研究成果報告書概要"},{"bibliographic_title":"1991 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"単クロ-ン抗体による免疫組織染色により胎盤型ALPは癌特異性の高いことが判明したため,遺伝子レベルでの発現について検討を試みた。胎盤型ALPを産生している新鮮胎盤組織と絨毛上皮癌BeWo細胞株を陽性対象として,胃癌KATOーIII細胞ならびに小腸粘膜組織よりRNAを抽出し,RTーPCR法を用いて胎盤型ALPmRNAの発現の有無について検討を行った。すなわち,抽出した各RNAにrandom hexamerと逆転写酵素を用いて,cDNAを作成した。胎盤型ALPと小腸型ALPのcDNA塩基配列の比較により,胎盤型ALPに特異的と考えられる領域(214bp)をはさみ込む25oligonucleotideをDNA合成した。これをprimerとして用いて,RNAより変換されたcDNA中の胎盤型ALPcDNAの特異領域(214bp)を増幅し,胎盤型ALPmRNAの発現の有無について検索した。その結果,胎盤組織,KATOーIII,BeWo細胞株に胎盤型ALPmRNAの発現が認められたが,小腸粘膜組織にも214bpの胎盤型ALPcDNAの出現が認められた。最近胎盤型ALPのgenomic DNAの塩基配列が解明され設定していた214bpは,exon 9,10,11から構成されており,特異的と考えられたこの領域も,他領域と同様にexon,intron共に,小腸型ALPと非常に類似していることが判明した。1にて,胎盤型ALPの蛋白レベルでの発現は,胃腸上皮化生では認められなかったことより,今回のRTーPCR法の結果は,小腸粘膜組織に胎盤型ALPが発現されているというよりも,小腸型ALPmRNAにも交差反応を示した可能性が強いと思われた。また,KATOーIII細胞では胎盤型ALPcDNAのbandが214bpよりも小さなbandとして出現しており,特異領域内の一部の塩基が欠失している可能性が考えられた。現在,これらの点を確認するために,dideoxy法によるDNA sequencingにより塩基配列を決定するなどの実験を継続中である。","subitem_description_type":"Abstract"},{"subitem_description":"Intestinal metaplasia of the gastric mucosa has been suggested to represent a precancerous state. However, how it is related to carcinogenesis remains controversial. We developed specific monoclonal antibodies (MAbs) against placental alkaline phosphatase (PLAP). Imnninohistochemical study with the specific MAb showed that expression of PLAP was apt to occur in more highly differentiated gastric carcinoma, and was highly specific for carcinoma. Therefore, we attempted to elucidate the expression of PLAP MRNA with the RT-PCR method in various benign and malignant gastric diseases. Each RNA was extracted from KATO-III cells (gastric cancer), intestinal mucosal tissue, BeWo cells (chorionic carcinoma), and placental tissue. Primer pairs were selected as synthetic 25 oligonucleotides which detected the PLAP cDNA region (2l4 bp) which was widely different from intestinal alkaline phosphatase cDNA (IAP cDNA). The results of RT-PCR showed that 214 bp band appeared in samples from KATO-III cells, BeWo cells, intestinal mucosal tissue as well as placental tissue. On the other hand, PLAP cDNA is very similar to IAP cDNA. These results indicated that primer pairs probably reacted with both PLAP mRNA and IAP mRNA. Futhermore, the amplified PLAPcDNA region in the sample of KATO- III was recognized as a slightly small weight band in electrophoresis and therefore several base pairs of the amplified region might be deleted in KATO- III cells. To clarify these points, further investigations continue.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:02670301, 研究期間(年度):1990 – 1991","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「胎盤型アルカリフォスファタ-ゼのmRNAの発現からみた胃腸上皮化生と胃癌との関連」課題番号02670301\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02670301/026703011991kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学がん研究所","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067328","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60160660"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60160660","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02670301/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-02670301/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02670301/026703011991kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-02670301/026703011991kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-27"}],"displaytype":"detail","filename":"CA-PR-OKAI-T-kaken 1993-2p.pdf","filesize":[{"value":"97.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"CA-PR-OKAI-T-kaken 1993-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61084/files/CA-PR-OKAI-T-kaken 1993-2p.pdf"},"version_id":"38927522-bf17-48df-b9a7-e399668ba9f6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"胎盤型アルカリフォスファタ-ゼのmRNAの発現からみた胃腸上皮化生と胃癌との関連","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎盤型アルカリフォスファタ-ゼのmRNAの発現からみた胃腸上皮化生と胃癌との関連"},{"subitem_title":"Relationship between gastric carcinoma and intestinal metaplasia from the aspect of the expression of placental alkaline phosphatase messenger RNA","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2842"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-27"},"publish_date":"2022-10-27","publish_status":"0","recid":"61084","relation_version_is_last":true,"title":["胎盤型アルカリフォスファタ-ゼのmRNAの発現からみた胃腸上皮化生と胃癌との関連"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:02:22.458418+00:00"}