{"created":"2023-07-27T07:01:03.439830+00:00","id":61199,"links":{},"metadata":{"_buckets":{"deposit":"7676fbf5-f151-436e-8386-a36ac250a392"},"_deposit":{"created_by":18,"id":"61199","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61199"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061199","sets":["2812:2813:2840"]},"author_link":["107758","20437"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03-26","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1991 – 1993","bibliographic_titles":[{"bibliographic_title":"平成5(1993)年度 科学研究費補助金 一般研究(A) 研究成果報告書概要"},{"bibliographic_title":"1993 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒトTリンパ球はCD45抗原ファミリーの発現を指標に、ナイーブT細胞、メモリーT細胞に大別されることが知られてきた。ヒト新生児T細胞の殆どはCD45RA^+ナイーブT細胞からなるが、生後、様々な抗原刺激によりCD45RA^+からCD45RO^+への形質変換、機能的成熟を遂げ、成長につれ特異的免疫能を獲得したメモリーT細胞プールが増大すると考えられる。伝染性単核症をモデルに、ウイルス感染時のT細胞の活性化と増殖、それに引き続く特異的なメモリーT細胞の成立の機構を解析した。\n1)伝染性単核症T細胞を免疫原として、IMN3・1単クローン抗体を作成した。この抗体は、伝染性単核症などにみられる活性化細胞、AIDSのCD4^+T細胞、胸腺細胞など、アポトーシスに陥り易いT細胞に選択的に発現する新しいT細胞後期活性化抗原(分子量約120kDa)を認識する。\n2)伝染性単核症急性期には、活性化を受け、CD45RO抗原を発現した異型リンパ球の著増する。このような活性化T細胞はFas/Apo-1抗原を発現し、アポトーシスに陥り易く、疾患の経過とともに急速に除かれる。\n3)正常人のCD45RO^+メモリーT細胞にもFas抗原の発現がみられるが、伝染性単核症のCD45RO^+T細胞と異なり、アポトーシス抵抗性である。前者にはbcl-2蛋白の細胞内発現がみられるが、後者にはbcl-2蛋白の発現はみられない。同じくFas抗原を発現した細胞でも、bcl-2発現の強弱によりアポトーシス感受性に差がある。一般に、T細胞の活性化に伴いbcl-2蛋白の発現減弱、Fas抗原の発現増強がみられ、アポトーシス感受性は亢進するが、なんらかの機構でFas^+/bcl-2^+の形質を獲得した一部のT細胞が細胞死を免れ、T細胞メモリーが成立するものと考えられる。\n4)Fas抗原は末梢リンパ球の一部、単球、好中球に発現がみられるが、Bcl-2発現はリンパ球、単球、好中球の順に減弱し、それぞれの細胞群のアポトーシス抵抗性と密接に関係するもののようである。","subitem_description_type":"Abstract"},{"subitem_description":"Immune stimulation of T cells resulted in the conversion CD45 antigen expression form CD45RA 'naive' to CD45RO 'primed' phenotype. It has been suggested that memory T cells reside within CD45RO 'primed' T CELL POOL.As T cells response during EBV-induced infectious mononucleosis is particularly intense. dynamics in the acquisition and generation of T cell memory was evauated largely in this disease model.\n1. Monoclonal antibody IMN3.1, prodced with T cells in the acute phase of infectious mononucleosis as imunogen, identified a novel T cell activatin antigen, which was expressed preferentially on apoptosis-prone T cells, such as mononucleosis T cels, thymocytes, and anti-Fas-sensitive T cell lines, but not on resting circulating T cellls.\n2. Atypical lymphocytes in infectious mononuchlosis are activated CD45RO^+ T cells. The majority of these stimulated T cells seemed to be eliminated in vivo by apoptotic cel death.\n3. CD45RO^+ T cells in infectious mononucleosis expressed Fas as well as IMN3.1 antigen and were sensitive to apoptotsis. In contrast, CD45RO^+ T cells from healthy individuals expressed Fas, but not IMN3.1, antigen, and were resistant to apoptosis. CD45RO^+ blastoid T cells in mononucleosis expressed no detectable leels of bc1-2 protein, whereas CD45RO^+ T cells in healthy subjects were seemingly a quiescent state and expressed considerable levels of bc1-2. The reduced expression of bc1-2 on the majority of stimulated Tcells might render these cells rone to apoptosis. Onyly a few of stimulated T cells expressing both Fas and bcl by unclear mechanisms, however, might be rescued from cell death and might re-enter to a quiescent state as specified functional memory T cells\n4. Apoptotic tendency in vitro aging of lymhocytes, monocytes, and neutrohils was in increasing order of sensitivity and seeed to be inversely correlaed with their expression levels of bc1-2.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:03404034, 研究期間(年度):1991 – 1993","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「小児における特異的免疫能の成立とメモリーT細胞の動態に関する研究」課題番号03404034\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03404034/034040341993kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067443","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10019888"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10019888","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-03404034/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-03404034/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03404034/034040341993kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03404034/034040341993kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-24"}],"displaytype":"detail","filename":"ME-PR-TANIGUCHI-N-kaken 1995-2p.pdf","filesize":[{"value":"92.9 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TANIGUCHI-N-kaken 1995-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61199/files/ME-PR-TANIGUCHI-N-kaken 1995-2p.pdf"},"version_id":"20e5618c-3137-4d7f-985a-b6157907b1af"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"小児における特異的免疫能の成立とメモリーT細胞の動態に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"小児における特異的免疫能の成立とメモリーT細胞の動態に関する研究"},{"subitem_title":"Acquisition of Specific Immunity and Dynamics in the Generation of Memory T Cells in Children","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2840"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-24"},"publish_date":"2022-10-24","publish_status":"0","recid":"61199","relation_version_is_last":true,"title":["小児における特異的免疫能の成立とメモリーT細胞の動態に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:04:52.613631+00:00"}