{"created":"2023-07-27T07:01:03.939707+00:00","id":61210,"links":{},"metadata":{"_buckets":{"deposit":"2b80d55d-e23f-4eaf-9b9b-7411fa49d99d"},"_deposit":{"created_by":18,"id":"61210","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61210"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061210","sets":["2812:2813:2841"]},"author_link":["105578","21298"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1991 – 1992","bibliographic_titles":[{"bibliographic_title":"平成4(1992)年度 科学研究費補助金 一般研究(C) 研究成果報告書概要"},{"bibliographic_title":"1992 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"小腸移植の適応となる短腸症候群の症例ではしばしば肝障害がみられ,小腸と肝臓の同時移植が必要な症例が多く,また,臨床的には小腸と肝臓が同時に移植された症例では小腸単独の移植症例より免疫抑制が容易であるとされている。移植腸管で分泌される免疫グロブリンや免疫系細胞は肝臓に流入するため,小腸と肝臓の同時移植例では抗原性や免疫応答が変化する可能性がある。また,小腸移植どはgraftのパイエル板や腸間膜リンパ節に多くのリンパ球を含む。そこで,術後の腸管の粘膜防御機能の変化と細胞性免疫機能に重要な役割を果たすリンパ球の動態を検討した。ラットを用いて,Lewis-Brown Norway F1 hybrid(LBNF1)をdoner,Lewisをrecipientとした群をrejection(RJ)群,Lewisをdoner,LBNF1をrecipientとした群をGVHD群,GVHD群に0.32mg/kg/dayのFK-506を投与した群をFK群,isograft群をcontrol群とした。GVHD群ではgraftのPeyer板のBrdU labellingindexは30.6±9.2と有意に高値を示し,RJ群ではnativeの腸間膜リンパ節のDNA合成時間は有意に延長していた。RJ群ではgraftのIgGは高値をIgMは低値を示し,GVHD群ではgraftとnative腸管のIgG,graftのIgMは増加したが,native腸管のIgMとglucosamineは減少した。FK群ではIgCとglucosamineの変化は抑制されたが,IgMの低下は防止されなかった。BrdUをトレーサーとしたリンパ球動態の検討では,GVHD群のgraftからrecipientの肝臓と脾臓へのリンパ球の移行が認められ,脾臓のW3/25陽性細胞は移植後14日目で有意に増加した。さらに血液中のW3/25陽性細胞は移植後3日目に一旦減少した後,14日目には有意に増加することを明らかにした。","subitem_description_type":"Abstract"},{"subitem_description":"Purebred male Lewis (Lew) and LewisXBrown Norway F1 hybrid (LBNF1) rats weighting 200-300 g were used. The heterotopic small bowei transplantation was carried out using the technique of Monchik and Russel. For the first part of investigation, the tracing of lymphatic cells from grafts was performed. The small bowel was dissected as a graft after intra-peritoneal injection of 20 mg of bromodeoxyuridine (BrdU). The heterotopic small bowel transplantation was carried out in GVHR group (Lew rats served as doner, and LBNF1 rats as recipient). The animals were sacrificed on the fifth day after transplantation. The mucosal specimens and mesenteric lymph nodes from the native intestine were obtained. The sections were stained with ABC method. Anti-Br dU antibody and anti-surface markers of lymphocytes (W3/13) were used.\nFor the second part of investigation, the animals were divided in two groups to investigate the immunological cell kinetics. The first was iso graft group as a control. LBNF1 rats served as doner and recipient. The second was rejection group. LBNF1 rats served as doner and Lew served as recipient. The third was of GVHR. Lew rats served as doner, and LBNF1 rats as recipient. The animals were sacrificed on the eighth day after administration of 20 mg of Br dU. Br dU labelling index and DNA synthesis time of both Peyer's patch and mesenteric lymph node were examined with flowcytometer.\nWe documented that the specimens from the native mesenteric lymph nodes include some Br dU positive lymphocytes that immigrated from the graft in GVHR. There was a marked increase of W3/13 positive cells in lymph nodes in GVHR These allogenic T lymphocytes become stimulated and develop an immune response against the native intestine4). We also documented that the Br dU labeling index and DNA synthesis time demonstrated that the lymphatic system of the graft was stimulated, but those of the native intestine was strongly suppressed in GVHR.\nThese findings suggest that the diffuse barrier function of the mucosal linigs of the native intestine had weakened in GVHR. This results in bacteria and antigens breaking into the portal system, and sometimes causing recipients to develop a septic condition. The combined (small bowel and liver) transplantation may be useful for small bowel transplantation to decrease antigenesty of small bowel graft.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:03670610, 研究期間(年度):1991 – 1992","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「小腸肝同時移植後のGVHDに関する研究」課題番号03670610\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03670610/036706101992kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部附属病院","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067454","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=00182303"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=00182303","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-03670610/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-03670610/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03670610/036706101992kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-03670610/036706101992kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-24"}],"displaytype":"detail","filename":"HO-PR-YAGI-M-kaken 1994-2p.pdf","filesize":[{"value":"85.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"HO-PR-YAGI-M-kaken 1994-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61210/files/HO-PR-YAGI-M-kaken 1994-2p.pdf"},"version_id":"37fd29a4-2969-4781-bdef-aba2d8e8fa14"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"小腸肝同時移植後のGVHDに関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"小腸肝同時移植後のGVHDに関する研究"},{"subitem_title":"Gvhr in combined small bowel and liver transplantation","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2841"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-24"},"publish_date":"2022-10-24","publish_status":"0","recid":"61210","relation_version_is_last":true,"title":["小腸肝同時移植後のGVHDに関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T12:05:09.840060+00:00"}