{"created":"2023-07-27T07:01:15.115832+00:00","id":61468,"links":{},"metadata":{"_buckets":{"deposit":"43603aa4-4557-4e96-b5ec-121213639c67"},"_deposit":{"created_by":18,"id":"61468","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61468"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061468","sets":["2812:2813:2845"]},"author_link":["27584","108135"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-03-19","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1987 – 1988","bibliographic_titles":[{"bibliographic_title":"昭和63(1988)年度 科学研究費補助金 一般研究(C) 研究成果報告書概要"},{"bibliographic_title":"1988 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Masamune, Yukito"}],"nameIdentifiers":[{"nameIdentifier":"27584","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00013318","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00013318"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"2つのホスホグリセリン酸キナーゼ(PGK1、PGK2)遺伝子の精子形成過程での発現切り換わり機構の解明を目的として研究を行い、以下に述べる知見を得た。\n1.PGK1、PGK2遺伝子の構造解析\n両刷伝子を含むマウスゲノムDNAクローンをそれぞれ単離し、制限酵素地図の作製および部分塩基配列の決定を行った。PGK2遺伝子はすでに報積されたようにイントロンを含まない遺伝子であった。PGK1遺伝子は複数のエクソンから成っていた。PGK2遺伝子の転写開始点は近接した7つの部位に存在し、その上流域にはTATAボックス構造は認められず複数のCAATボックスおよびGCボックスが存在する。PGK1遺伝子のプロモーター構造については現在解析中である。\n2.精子形成過程での2つのPGK遺伝子転写の切り換わり時期の決定\nマウス精巣切片を用いたin situハイブリダイゼーションにより、2つのPGKmRNAが分化過程のどの時期の細胞に存在するかを解析した。PGK1mRNAは非生殖細胞およびspermatogonia、spermatocytesに検出されたが、ハプロイドとなったspermatidsでは消失していた。PGK2mRNAは減数分裂開始後のspermatocytesにはじめて出現し、spermatidsの後半で検出されなくなった。従って、PGK刷伝子転写のPGK1からPGK2への切り換わりは減数分裂開始後の4nとなったspermatocytesで起こると考えられる。また、転写と合わせて翻訳段階での制御もPGK遺伝子発現の切り換わりに重要であると予想される。\n今後両PGK遺伝子プロモーターの機能解析を行い、精子形成過程での転写切り換わり機構に関する遺伝子側の要素およびトランスに働く因子を同定したいと考える。","subitem_description_type":"Abstract"},{"subitem_description":"1. Structural analysis of two phosphoglycerate kinase genes. Genomic DNA clones containing the two phosphoglycerate kinase (PGK1,PGK2) genes have been isolated and their restriction-enzyme maps and partial nucleotide sequences were determined. The PGK2 gene was found to have no intron as previously reported. The PGK1 gene consists of multiple exons. Transcriptoon start-site of the PGK2 gene was multiple, and no TATA-box was present in the 5'-upstream region of the gene and several CAAT-boxes and GC-boxes exist instead. Structure of the PGK1 gene promoter remains to be determined.\n2. In situ localization of two PGK mRNAs in mouse testis. The localization of the two PGK mRNAs was determined with mouse testis sections by in situ hybridization. PGK mRNA was detected in non-germinal Leydig and Sertoli cells, and inspermatogonia and spermatocytes early in spermatogenesis, but it desappeared in spermatids. PGK2 mRNA became first detectable in spermatocytes of later stage and disappeared in late spermatids. These results suggest the switch of the PGK gene transcription to occur at the spermatocyte stage after the onset of meiosis. Regulation at the translation in addition to transcription step may possibly be required for the switch of the PGK gene expression during mammalian spermatogenesis.\nFuncrional analysis of the promoters of the two PGK genes should be done so as to identify cis-elements and trans-acting factors responisble for the switch of the two PGK gene transcription during spermatogenesis.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:62570980, 研究期間(年度):1987 – 1988","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「マウスホスグリセリン酸チナーゼ遺伝子の組織特異的発現機構の解析」課題番号62570980\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-62570980/625709801988kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学薬学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067711","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/search/?kw=00013318"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/search/?kw=00013318","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-62570980/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-62570980/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-62570980/625709801988kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-62570980/625709801988kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"正宗, 行人"}],"nameIdentifiers":[{"nameIdentifier":"108135","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00013318","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00013318"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-31"}],"displaytype":"detail","filename":"PH-PR-MASAMUNE-Y-kaken 1990-2p.pdf","filesize":[{"value":"89.3 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-MASAMUNE-Y-kaken 1990-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61468/files/PH-PR-MASAMUNE-Y-kaken 1990-2p.pdf"},"version_id":"a9e8815d-8353-4f92-b44f-a6ffe4b54a1f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"マウスホスグリセリン酸チナーゼ遺伝子の組織特異的発現機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウスホスグリセリン酸チナーゼ遺伝子の組織特異的発現機構の解析"},{"subitem_title":"Analysis of tissue-specific expression of two phosphoglycerate kinase genes.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2845"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-10-31"},"publish_date":"2022-10-31","publish_status":"0","recid":"61468","relation_version_is_last":true,"title":["マウスホスグリセリン酸チナーゼ遺伝子の組織特異的発現機構の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:53:20.401214+00:00"}