{"created":"2023-07-27T07:01:24.258481+00:00","id":61690,"links":{},"metadata":{"_buckets":{"deposit":"6c377614-7049-4b7c-9178-2492c1e5d494"},"_deposit":{"created_by":18,"id":"61690","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61690"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061690","sets":["2812:2813:2846"]},"author_link":["27548","92309"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-03-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1986 – 1987","bibliographic_titles":[{"bibliographic_title":"昭和62(1987)年度 科学研究費補助金 一般研究(A) 研究成果報告書概要"},{"bibliographic_title":"1987 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒトを含む哺乳類細胞を同調し, 紫外線(UV)を照射すると, 他の細胞周期に比べて, S期における照射が最も高い細胞がん化と突然変異誘発効果を示した. 一方DNA複製に異常を示す色素性乾皮症亜型(XPV)患者由来細胞でもS期におけるUV照射で同様な生物学的効果がもたらされることが知られている. 本研究ではS期に照射された細胞DNAに生じた損傷とその消長を極めて鋭敏に, かつ1ヶ1ヶの細胞について定量することを目的とした. そのために種々のDNA損傷を特異的に認識するモノクローン抗体を作製することとした.\nまず第1に, 大線量のUVを照射したDNAを抗原としてマウスを免疫し, 脾細胞を取り出して骨髄腫細胞と融合したところ, 多数の融合細胞中にUV照射DNAを抗原として結合する抗体産生クローンを得た. その後の研究で, 本抗体はチミンーチミン, チミンーシトシン配列を持つ(6-4)光産物を認識することが判明したため, 本抗体を64M-1と命名した. ついで, 313nmのUVをアセトフェノン存在下で照射されたDNAを抗原としてマウスを免疫した結果, 融合細胞中よりチミン2量体と特異的に結合する抗体(TDM-1)産生細胞を分離した.\n64M-1, TDM-1抗体を用いて, 正常ヒト, 色素性乾皮症(XP), XPV細胞中のUV損傷の除去動態を調べたところ, 正常ヒト細胞では(6-4)光産物はチミン2量体より早く除去されるが, XP細胞では両者の除去は認められなかった. また, XPV細胞ではチミン2量体の除去は正常であるが, 6-4光産物の除去は正常細胞に比べ低いことが判明した. この6-4光産物の除去能の低下が, XPV細胞における高い発がん, 突然変異, 誘発効果を招来している可能性が示された.","subitem_description_type":"Abstract"},{"subitem_description":"Previously we reported that the cells irradiated with ultraviolet (UV) light at S phase revealed the highest transformation and mutation frequencies over various cell phases. In order to understand the mechanisms underlying these high frequencies, we developed an immunological method allowing us to follow the excision kinetics of UV-damage in single cells. Consequently, we established two monoclonal antibodies differentially recognizing each specified UV-damage.\nAt first, the hybridomas between spleen cells from a mouse immunized with UV-DNA and myeloma cells were screened in their binding to UV-DNA and a hybridoma producing antibody against (6-4) photoproduct in DNA was isolated. A further characterization of the antibosy, 64M-1 revealed that it recognizes (6-4) photoproduct of thyminethymine or thymine-cytosine sequence in UV-DNA.\nSecondary, by the fusion between the spleen cells from a mouse immunized with 313 nm UV-DNA in the presence of acetophenone, a hybridoma secreting antibody directed against thymine-thymine dimer was isolated. The antibody TDM-1 bond to DNA irradiated with 313 nm UV in the presence of acetophenone and to UV-irradiated oligo(dT)_8.\nThe excision of (6-4) photoproduct and thymine dimer in UV-irradiated normal human, xeroderma pigmentosum(XP) and its variant (XPV) cells were compared by the enzyme linked immunosorbent assay(ELISA) using two monoclonal antibodies. The results obtained so far indicate that (1) (6-4) photoproduct was excised from DNA faster than thymine dimer in normal cells, (2) XP cells excised neither (6-4) photoproduct nor thymine dimer, and (3) XP cell were deficient in the excision of (6-4) photoproduct but proficient in the excision repair of thymine dimer. The defective repair of (6-4) photoproduct in XPV cel may explain their highly mutable and transfomable nature.\nThe labelling of the 64M-1 and TDM-1 antibodies with radioisotopes or fluorescence dyes made it possible to reace the excision kinetics of (6-4) photoproduct and thymine dimer in single cell. The experiment to follow the excision kinetics of such damage given at S phase in a cell through various phases is now in progress.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:61440091, 研究期間(年度):1986 – 1987","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「DNA複製による放射線損傷の固定とその効果」課題番号61440091\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-61440091/614400911987kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学薬学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00067933","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=60019669"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=60019669","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-61440091/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-61440091/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-61440091/614400911987kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-61440091/614400911987kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-10"}],"displaytype":"detail","filename":"PH-PR-NIKAIDO-O-kaken 1989-2p.pdf","filesize":[{"value":"105.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-NIKAIDO-O-kaken 1989-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61690/files/PH-PR-NIKAIDO-O-kaken 1989-2p.pdf"},"version_id":"b6b9a3a8-4401-452c-b045-624359700b50"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"DNA複製による放射線損傷の固定とその効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA複製による放射線損傷の固定とその効果"},{"subitem_title":"Expression and fixation of ultraviolet light-induced DNA damage through cell cycle progression of human cells in culture.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2846"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-10"},"publish_date":"2022-11-10","publish_status":"0","recid":"61690","relation_version_is_last":true,"title":["DNA複製による放射線損傷の固定とその効果"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:42:37.352674+00:00"}