{"created":"2023-07-27T07:01:28.345632+00:00","id":61794,"links":{},"metadata":{"_buckets":{"deposit":"52387828-147d-4a50-ae9f-eee42b753181"},"_deposit":{"created_by":18,"id":"61794","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61794"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061794","sets":["2812:2813:2846"]},"author_link":["26530"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-03-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"4p.","bibliographicVolumeNumber":"1985 – 1987","bibliographic_titles":[{"bibliographic_title":"昭和62(1987)年度 科学研究費補助金 一般研究(A) 研究成果報告書概要"},{"bibliographic_title":"1987 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"高度焼損死体の個人識別および死因究明上における法医診断学的諸問題の解決を目的として本研究は行われ, 以下の新知見が得られた.\n1.ヒト組織細胞の熱変化と血液活性\n(1)血球浮遊液を加熱すると100°CではABO.Lewis.MNS-S.Rh-Hr型など検査したすべての活性が残存, 180〜200°C加熱でもABO.Lewis型活性は検出し得た. 電顕上, 加熱(60〜100°C)により血球膜の膨化や断片所見がみられた. 塊状に変化した膜関連物質にABO型活性を認めた(フェリチン標識法). (2)ヒト組織細胞内型活性を, 光顕レベルでの所見と対比しながら電顕的(コロイド金標識法)に検出し, 粘液細胞の分泌顆粒や血管内皮細胞に型活性を確認. (3)加熱ヒト組織では, 炭化直前層は電顕的にも無構造化に傾く. 深部の光顕的細胞構造は一見保たれているが, 電顕的に細胞膜の膨化, 断つ裂や基質の集塊化がみられ, ABO型活性を血管内皮や粘液細胞内に検出し得た. (4)血液型物質関連単クローン抗体として種属特異的抗赤血球膜抗体, ヒト唾液物質特異的抗体の作製に成功した.\n2.高度焼損死体の法医中毒学的研究\n(1)揮発性並びにガス状薬毒物について, 熱凝固した卵白塊内に添加エタノールやトルエンが検査部位の熱変化程度に応じて残存することが基本的に確認された. 焼死の動物モデル実験により, 上記薬毒物並びにプロパン, エーテル, クロロホルムが強く焼けた臓器からでも検出し得ることがGCおよびGC-MS法により明らかにされた. また,キュリーポイント・パイロライザーを応用した微量試料からの発揮性薬毒物測定法を新しく開発し得た. (2)クロルプロマジン, ジアゼパムや覚醒剤が炭化直前の組織試料からでもGC-MS法で分析可能であることが示された.","subitem_description_type":"Abstract"},{"subitem_description":"This research has been carried out in order to offer solutions of the following medicolegal problems concerning severely charred bodies; personal identification and diagnosis of cause of death.\n1. Thermo-changes of human tissue cells and their blood group activities:\nAll the activities examined in the systems such as the ABO, Lewis, MNSs and Rh remained on the denaturated red cells after heating at 100゜c. The ABO and Lewis group activities were detectable after heating at 180-200゜c. Electron-microscopically, the thermodenaturated cells (60-100゜c) showed swelling and fragmentation of the membranes. By immunoferritin staining, the ABO group activities were demonstrated on the membranerelated substances which showed a coagulative change.\nUltrastructural localization of the ABO group activities in some kinds of cells such as mucous cells and endothelial cells of the vessels were shown by immunogold method. Nearly charred layuers of tissues were micro- and ultramicroscopically almost amorphous. Inner parts, retaining the microscopic structures fairly well, ultramicroscopically showed swelling and fragmentation of the cell membranes and a coagulative change of the matrix, and the ABO group activities were detectable in the cells above. Monoclonal antibodies could be produced against blood group antigen-related substances; anti-human red cell membrane and anti-human salivary blood group substance.\n2. Forensic toxicological studies on severely charred bodies:\nOn volatile and gaseous poisons, fundamental experiments showed that ethanol and toluene remained in charred egg white masses, their contents being correlated with the thermochanges of the portions examined. In the experiments using animal carcasses, it was shown that ethanol, toluene, propane, ether and chloroform were detectable from charred organs. for the analysis of volatile poisons from traces of specimens, a new method was developed employing a curie-point pyrolyser.\nChlorpromazin, diazepam and methamphetamine proved to be determinable by GC-MS from nearly charred tissue specimens.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:60440041, 研究期間(年度):1985 – 1987","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「高度焼損死体に関する法医学的研究」課題番号60440041\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60440041/604400411987kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医学部法医学講座","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00068037","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20073679"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20073679","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60440041/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60440041/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60440041/604400411987kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60440041/604400411987kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-10"}],"displaytype":"detail","filename":"ME-PR-NAGANO-T-kaken 1989-4p.pdf","filesize":[{"value":"149.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-NAGANO-T-kaken 1989-4p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61794/files/ME-PR-NAGANO-T-kaken 1989-4p.pdf"},"version_id":"dfa65a0d-29eb-4faf-8747-1b824b81bf45"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"高度焼損死体に関する法医学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"高度焼損死体に関する法医学的研究"},{"subitem_title":"Medicolegal studies on severely burned bodies.","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2846"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-10"},"publish_date":"2022-11-10","publish_status":"0","recid":"61794","relation_version_is_last":true,"title":["高度焼損死体に関する法医学的研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:44:06.566686+00:00"}