{"created":"2023-07-27T07:01:28.527822+00:00","id":61798,"links":{},"metadata":{"_buckets":{"deposit":"62e2cf4b-b0c2-4b34-bead-89aaa6438ab5"},"_deposit":{"created_by":18,"id":"61798","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61798"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061798","sets":["2812:2813:2847"]},"author_link":["26399","20437"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1988-11-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1985 – 1986","bibliographic_titles":[{"bibliographic_title":"昭和61(1986)年度 科学研究費補助金 一般研究(B) 研究成果報告書概要"},{"bibliographic_title":"1986 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{}],"nameIdentifiers":[{},{}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1.ヒト胎児末梢血のNK細胞活性は、姙娠後3半期にいたり出現し、姙娠月数の進むにつれ増強するが、その活性は生下時にはなお弱く、成人対照の1/2〜1/3に止まる。リンホカイン応答性についてみると、インターフェロン・ガンマ(IFN-γ)によるNK細胞活性の増強は、basal存NK細胞活性に依存し、NK細胞活性を欠く胎生20週令では、IFN-γによるNK細胞活性の誘導はみられないが、インターロイキン・2(IL-2)により、IFN-γ産生を介することなく、強にNK細胞活性が誘導される。また、新生児Leu【11^+】細胞のNK細胞活性は低いが、IL-2により成人対照レベルにまで増強され、さらに、basalなNK細胞活性を欠く新生児Leu【11^-】細胞群にも強いNK細胞活性が誘導されてくる。このような所見から、IFN-γ反応性NK細胞は、かなり分化は進んでいるが、機能的にはなお未熟なNK細胞と考えられ、IL-2反応性細胞は、より幼弱なNK前駆細胞であろうと推論された。\n2.新生児期にはLeu【11^+】細胞は成人レベルに近いが、HNK-【1^+】細胞に乏しい。新生児期の抗【CD_3】誘導性細胞障害能が極めて微弱なことは、HNK-【1^+】細胞に乏しい現象と深く関連するもののようである。\n3.新生児期のPHA誘導性IFN-γ産生は不良であり、3-4才にいたり初めて成人レベルに達するが、放射線照射後のPHA刺激,OK-432刺激では、新生児リンパ球でも良好なIFN-γの産生がみられ、免疫組織学的にも確かめられた。新生児期のPHA誘導性IFN-γ産生の不良な原因として、PHA刺激により、IFN-γ産生に抑制的に仂く、この時期に特徴的なサプレッサー細胞の活性化が強く起っていることが明らかにされた。このようなサプレッサー前駆細胞は、【T4^+】ヘルパー形質をもち、放射線感受性であることが判明した。","subitem_description_type":"Abstract"},{"subitem_description":"1. Peripheral blood mononuclear cells(MNC) from fetuses of estimated gestational age of 20 wk lacked NK cell activity against K 562 target cells even after 18 hr-treatment with interferon- <gamma> (IFN- <gamma> ). Low, but significant levels of basal and IFN- <gamma> -inducible NK cell activity were observed in premature infants of 27-wk-gestation, with a progressive increase of these activities during the last trimester of pregnancy. Contrary to IFN- <gamma> , interleukin-2(IL-2) could induce a marked NK cell activity even in MNC from fetuses of 20-wk-gestational age and in Leu- <11^-> cell population of cord MNC, each of them lacked both basal and IFN- <gamma> -inducible NK cell activity. These ontogenic development and lymphokine responsiveness of human NK cell activity indicated that putative precursors of NK effector cells might be divided into IFN- <gamma> -responsive, more mature inactive forms of NK cells and into putative NK progenitors responding well to IL-2, but not to IFN- <gamma> , which might appear at an earlier stage of fetal development than IFN- <gamma> -responsive ones.\n2. In cord blood, Leu- <11^+> cells were comparable in number with adult controls, but Leu- <7^+> cells were very meager. Preliminary studies indicated that low levels of Leu- <7^+> cells in cord MNC might be responsible for the poor ability of cord blood in their anti-CD3-inducible cytotoxicity.\n3. Dissociated production of IL-2 and IFN- <gamma> , ample production of IL-2 and poor secretion of IFN- <gamma> , on PHA stimulation is a characteristic of cord MNC. In this report, some experimental data suggesting that IFN- <gamma> production by PHA-stimulated cord MNC to be down-regulated by preferential activation of suppressor precursors against IFN- <gamma> production, were presented. These PHA-inducible suppressor precursors in cord MNC expressed in large T4 helper phenotype and were radiosensitive in nature.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:60480242, 研究期間(年度):1985 – 1986","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「胎生期・乳児期のNK細胞の分化とリンホカイン応答性に関する研究」課題番号60480242\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480242/604802421986kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大医学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00068041","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=10019888"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=10019888","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60480242/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60480242/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480242/604802421986kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480242/604802421986kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-07"}],"displaytype":"detail","filename":"ME-PR-TANIGUCHI-N-kaken 1988-2p.pdf","filesize":[{"value":"87.4 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-TANIGUCHI-N-kaken 1988-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61798/files/ME-PR-TANIGUCHI-N-kaken 1988-2p.pdf"},"version_id":"efbee515-d2b9-4cd8-94cd-9d774aaf49af"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"胎生期・乳児期のNK細胞の分化とリンホカイン応答性に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎生期・乳児期のNK細胞の分化とリンホカイン応答性に関する研究"},{"subitem_title":"Differentiation and Lymphokine Responsiveness of Putative NK Cells in Early Human Development","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2847"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-07"},"publish_date":"2022-11-07","publish_status":"0","recid":"61798","relation_version_is_last":true,"title":["胎生期・乳児期のNK細胞の分化とリンホカイン応答性に関する研究"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2023-07-27T11:45:57.752741+00:00"}