{"created":"2023-07-27T07:01:28.664179+00:00","id":61801,"links":{},"metadata":{"_buckets":{"deposit":"67d66b70-a9a8-4d48-bc1d-6056f171f9ed"},"_deposit":{"created_by":18,"id":"61801","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"61801"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00061801","sets":["2812:2813:2847"]},"author_link":["107600"],"item_9_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1988-11-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"2p.","bibliographicVolumeNumber":"1985 – 1986","bibliographic_titles":[{"bibliographic_title":"昭和61(1986)年度 科学研究費補助金 一般研究(B) 研究成果報告書概要"},{"bibliographic_title":"1986 Fiscal Year Final Research Report Summary","bibliographic_titleLang":"en"}]}]},"item_9_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Norio"}],"nameIdentifiers":[{"nameIdentifier":"107600","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20082133","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20082133"}]}]},"item_9_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"6目29種のウニ卵ゼリーより70%エタノール抽出液を調整し、バフンウニ(ホンウニ目に属する)精子への呼吸促進活性を検討した結果、ホンウニ目に属する16種の卵ゼリー中に呼吸促進活性を示す物質の存在が示唆されたが、他の5目(ヤワラウニ目,ガンガゼ目,アルバシア目,タコノマクラ目,ブンブク目)に属するウニ卵ゼリーにはバフンウニ精子の呼吸促進を引き起こす活性は認められなかった。またホンウニ目に属するウニ卵ゼリーのバフンウニ精子呼吸促進活性の程度も種の属する科によってわずかな違いが認められた。これらの事実から、ウニ卵ゼリー中に存在する精子活性化ペプチドの構造には分類学上の\"目\"のレベルで大きな違いがあり、同じ目内の種でも生理活性上は大きな違いはなくても構造にはわずかな違いがあるペプチドの存在が予測され、それを実証した。ホンウニ目に属するウニにはsperact類(【Thr^5】-speract,【Ser^5】-speract,【Ser^(3,10)】-speract,【Ser^(3,5,7)】-speract,【Ser^1】,【Ala^3】,【Gly^5】-speract,【Ala^3】,【Gly^5】-speract,【Ser^3】,【Thr^5】-speract,【Ser^(3,5,7)】,【Asp^(10)】-speract,【Thr^5】,【Gln^(10)】-speract,Des-【Gly^1】-(【Thr^5】,【Gln^(10)】-speract)が存在し、アルバシア目のウニにはresactおよびalloresactが、タコノマクラ目のウニにはmosact類(Des-【Gln^6】,【Asn^7】)-(【His^6】)+mosact,Des-【Gln^6】,【Asn^7】-(【Phe^6】)-mosact)が存在する。これらの特異的精子活性化ペプチドはその作用時には精子細胞膜上に存在する特異的なレセプターに結合するが、speractを用いた実験より、そのレセプターは精子の尾部に局在することが明らかになった。精子尾部のレセプターに結合した特異的ペプチドは尾部細胞膜に局在する高分子タンパク質の低分子化を引き起こすことが調べた全ての種で確認された。この低分子化には【Na^+】の存在が不可欠であった。特異的ペプチドによる精子呼吸促進に【Na^+】が必須であることから、この低分子化と呼吸促進との関係を明らかにすることが今後の課題である。","subitem_description_type":"Abstract"},{"subitem_description":"Species specific sperm activating peptides were purified and their amino acid sequences were determined. Mosact (Asp-Ser-Asp-Ser-Ala-Gln-Asn-Leu-Ile-Gly) and its two derivatives were obtained from the egg jelly of the sea urchin Clypeaster japonicus. Alloresact (Lys-Leu-Cys- Pro-Gly-Gly-Asn-Cys-Val) and its five derivatives were from Glyptocidaris crenularis egg jelly. Many new speract derivatives were purified from the egg jelly of sea urchins Hemicentrotus pulcherrimus, Anthocidaris crassispina, Pseudocentrotus depressus, Strongylocentrotus purpuratus and Lytechinus pictus. They are <Ser^(3,10)> -speract, <Ser^1> , <Ala^3> , <Gly^5> -speract, <Ser^(3,5,7)> , <Asp^(10)> -speract, <Ser^(3,5,7)> -speract, <Ala^3> , <Gly^5> -speract, <Ser^3> , <Thr^5> -speract, <Thr^5> , <Gln^(10)> -speract and Des- <Gly^1> -[ <Thr^5> , <Gln^(10)> ]-speract. Speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) and its derivatives do not cross-react with mosact or alloresact and vice versa. Addition of mosact to C.japonicus spermatozoa resulted in the rapid appearance of a newly-stained protein on SDS-polyacrylamide gels as well as speract did to spermatozoa of sea urchins belonging to the order Echinoida and alloresact did to G. crenularis ( in the order Arbacioida ) spermatozoa. Sodium ions were essential for this.","subitem_description_type":"Abstract"}]},"item_9_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究課題/領域番号:60480022, 研究期間(年度):1985 – 1986","subitem_description_type":"Other"},{"subitem_description":"出典：研究課題「特異的ペプチドによる精子活性化の分子的機構」課題番号60480022\n（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） \n（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480022/604800221986kenkyu_seika_hokoku_gaiyo/）を加工して作成","subitem_description_type":"Other"}]},"item_9_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大理学部","subitem_description_type":"Other"}]},"item_9_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00068044","subitem_identifier_reg_type":"JaLC"}]},"item_9_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/search/?qm=20082133"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/search/?qm=20082133","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60480022/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-60480022/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480022/604800221986kenkyu_seika_hokoku_gaiyo/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-60480022/604800221986kenkyu_seika_hokoku_gaiyo/","subitem_relation_type_select":"URI"}}]},"item_9_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 範男"}],"nameIdentifiers":[{"nameIdentifier":"107600","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20082133","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20082133"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-11-07"}],"displaytype":"detail","filename":"SC-PR-SUZUKI-N-1988-2p.pdf","filesize":[{"value":"91.6 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-SUZUKI-N-1988-2p.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/61801/files/SC-PR-SUZUKI-N-1988-2p.pdf"},"version_id":"96f9fade-03c8-4dd0-b5a0-78f002d61502"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"特異的ペプチドによる精子活性化の分子的機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"特異的ペプチドによる精子活性化の分子的機構"},{"subitem_title":"Molecular mechanism of sperm activation by species specific egg jelly pepetides","subitem_title_language":"en"}]},"item_type_id":"9","owner":"18","path":["2847"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-07"},"publish_date":"2022-11-07","publish_status":"0","recid":"61801","relation_version_is_last":true,"title":["特異的ペプチドによる精子活性化の分子的機構"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-07-01T06:55:00.377696+00:00"}