{"created":"2023-07-27T06:24:47.227707+00:00","id":7836,"links":{},"metadata":{"_buckets":{"deposit":"ff0be3a7-70d9-4bfe-a712-a1a4a66819ea"},"_deposit":{"created_by":3,"id":"7836","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"7836"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00007836","sets":["934:935:936"]},"author_link":["10687","10689","10686","604","10685","10688","10690"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"805","bibliographicPageStart":"799","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"Bunseki Kagaku = 日本分析"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ダブシルクロリド（Dabs-Cl）は，高速液体クロマトグラフィーなどによるタンパク質分析の際のアミノ酸標識剤である．著者らは，Dabs-Clで標識された一連のダブシル化アミノ酸（Dabs-AA）を合成し，紫外可視吸収スペクトル及び円二色性（CD）スペクトルを測定し，Dabs-AAの水溶液中での会合挙動を検討した．希薄水溶液では，Dabs-AAの可視部の吸収はジメチルアミノアゾベンゼン色素特有のpH依存性を示す．Dabs-AAの濃度上昇に伴い，500 nm付近のDabs-AAのモノマー由来の吸収は減少し，360～400 nm領域にダイマーに由来するピークが観測される．ダイマーの形成は共溶媒として用いたジメチルスルホキシド濃度，溶液温度及びアミノ酸側鎖（R）に依存する．Dabs-L-Pheの363 nmに観測されるダイマーの吸収帯に対応して，379 nmに負の第一コットン効果，359 nmに正の第二コットン効果をもつ強い誘起分裂型CDスペクトルが観測された．これらの結果から水溶液中では，Dabs-L-Pheは負のキラリティーを示す配向でダイマーを形成していることが示唆された．　　　　A series of dabsylated amino acids (Dabs-AA) were prepared and their aggregation behavior in aqueous solution was studied by UV/Vis absorption and CD spectroscopies. Dabs-AAs show pH- dependent absorption in the visible region, characteristic of the dimethylamio azobenzene chromophore in a dilute aqueous solution. Upon increasing the concentration of Dabs-AAs, the visible absorption around 500 nm of the Dabs-AA monomer decreased its intensity, and a new band due to the Dabs-AA dimer appeared in the 360-400 nm region. Dimer formation depended on the DMSO co-solvent concentration, the solution temperature and the R-groups of amino acids. A split-type induced CD spectrum was observed for the dimer absorption band of Dabs-L- Phe at 363 nm: a negative first Cotton effect at 379 nm and a positive second Cotton effect at 359 nm. These results indicate that a pair of Dabs-L-Phe molecules were stacked with negative exciton chirality. © 2005 The Japan Society for Analytical Chemistry.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域物質化学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会 = Japan Society for Analytical Chemistry"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2116/bunsekikagaku.54.799","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.2116/bunsekikagaku.54.799","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jstage.jst.go.jp/article/bunsekikagaku/54/9/54_799/_article/-char/ja","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2005 by The Japan Society for Analytical Chemistry"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00222633","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0525-1931","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"林, 宏成"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐々木, 千鶴"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"国本, 浩喜"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"前田, 史郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井, 信造"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"桑江, 彰夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"花井, 一彦"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"TE-PR-KUNIMOTO-K-799.pdf","filesize":[{"value":"419.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"TE-PR-KUNIMOTO-K-799.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/7836/files/TE-PR-KUNIMOTO-K-799.pdf"},"version_id":"378b41f6-73c2-4676-a7e1-be867fdd0e82"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"水溶液中におけるダブシルアミノ酸の会合挙動","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"水溶液中におけるダブシルアミノ酸の会合挙動"},{"subitem_title":"Aggregation behavior of dabsylated amino acids in aqueous solution","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["936"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"7836","relation_version_is_last":true,"title":["水溶液中におけるダブシルアミノ酸の会合挙動"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T02:18:52.678024+00:00"}