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Intracellular Ca2+ Responses of 3D-Cultured Osteoblasts to Dynamic Loading
http://hdl.handle.net/2297/36912
http://hdl.handle.net/2297/369124b9c8667-7303-40d6-b7dc-72730db1aeb5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
TE-PR-TANAKA-S-318.pdf (842.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Intracellular Ca2+ Responses of 3D-Cultured Osteoblasts to Dynamic Loading | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Tanaka, Shigeo M.
× Tanaka, Shigeo M. |
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| 書誌情報 |
Journal of Biomechanical Science and Engineering 巻 7, 号 3, p. 318-327, 発行日 2012-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1880-9863 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1299/jbse.7.318 | |||||
| 出版者 | ||||||
| 出版者 | The Japan Society of Mechanical Engineers = 日本機械学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In bone cells, intracellular Ca2+ (iCa2+) functions as a second messenger in the mechanotransduction pathway. Its responses to mechanical stimulation can be observed microscopically on a rigid flat surface with a Ca2+ fluorescent indicator, generally, under fluid flow. However, bone cells are physiologically exposed to dynamic loading accompanied by bone matrix deformation. In this situation, microscopic methods of observing iCa2+ responses cannot be used because of the loss of focus or movement of cells out of the observation area. The purpose of this study was to develop a compact optical device for the observation of iCa2+ responses of osteoblasts to dynamic loadings accompanied by substrate deformation. This system comprised four light emitting diodes (LEDs) and a photodetector (PD) placed underneath a culture chamber, specifically designed for tissue-level iCa2+ observations. This device was used to study the frequency dependence of iCa2+ responses of osteoblasts to dynamic loading. MC3T3-E1 osteoblasts were cultured three-dimensionally in a collagen sponge scaffold with the fluorescent Ca2+ indicator Fluo-4 AM and mechanically stimulated by a 0.2% deformation of the sponge at 0.2, 2, or 20 Hz for 150 s. Our device succeeded in detecting temporal changes in the intensity of emitted fluorescent light, showing a frequency-dependent increase in fluorescence intensity. This device may contribute to further understanding of the mechanosensing and mechanotransduction mechanisms in bone under near-physiological conditions. | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2012 by The Japan Society of Mechanical Engineers 日本機械学会 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jsme.or.jp/index.html | |||||
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| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/jbse | |||||
