WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "0ada8963-09e9-439a-ade5-57e6089c9330"}, "_deposit": {"created_by": 3, "id": "26646", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "26646"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00026646", "sets": ["1763"]}, "author_link": ["44772", "20454", "44775", "44771", "44774", "44773", "97"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-06-15", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageEnd": "967", "bibliographicPageStart": "955", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "Prostate"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "BACKGROUND. Although paclitaxel is used for hormone-resistant prostate cancer, relapse definitely occurs later. Details of the molecular mechanism responsible for paclitaxel- resistance remain unclear. METHODS. We established paclitaxel-resistant cells, DU145-TxR and PC-3-TxR from parent DU145 and PC-3. To characterize these cells, we examined cross-resistance to other anticancer drugs. Expression of several potential genes that had been related to drug-resistance was compared with parent cells by RT-PCR and Western blotting. Methylation analysis of multiple drug resistance (MDR1) promoter was carried out using bisulfite-modified DNA from cell lines. Knockdown experiments using small interfering RNA (siRNA) were also performed to confirm responsibility of drug-resistance. Finally, cDNA microarray was performed to quantify gene expression in PC-3 and PC-3-TxR cells. RESULTS. The IC50 for paclitaxel in DU145-TxR and PC-3-TxR was 34.0- and 43.4-fold higher than that in both parent cells, respectively. Both cells showed cross-resistance to some drugs, but not to VP-16 and cisplatin. Methylation analysis revealed that methylated CpG sites of MDR1 promoter in DU145 and PC-3 cells were demethylated in DU145-TxR cells, but not in PC-3-TxR cells. Knockdown of P-glycoprotein (P-gp), which was up-regulated in resistant cells, by MDR-1 siRNA restored paclitaxel sensitivity in DU145-TxR but not in PC-3-TxR, indicating that upregulation of P-gp was not always main cause of paclitaxel-resistance. Microarray analysis identified 201 (1.34%) up-regulated genes and 218 (1.45%) out of screened genes in PC-3-TxR. CONCLUSIONS. Our data will provide molecular mechanisms of paclitaxel-resistance and be useful for screening target genes to diagnose paclitaxel sensitivity. © 2007 Wiley-Liss, Inc.", "subitem_description_type": "Abstract"}]}, "item_4_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学医学部附属病院泌尿器科", "subitem_description_type": "Other"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "John Wiley \u0026 Sons"}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1002/pros.20581", "subitem_relation_type_select": "DOI"}}]}, "item_4_source_id_11": {"attribute_name": "NCID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10621087", "subitem_source_identifier_type": "NCID"}]}, "item_4_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0270-4137", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takeda, Masashi"}], "nameIdentifiers": [{"nameIdentifier": "44771", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mizokami, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "97", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50248580", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=50248580"}, {"nameIdentifier": "50248580", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=50248580"}, {"nameIdentifier": "50248580", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000050248580"}]}, {"creatorNames": [{"creatorName": "Mamiya, Kiminori"}], "nameIdentifiers": [{"nameIdentifier": "44772", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "You, Qiang Li"}], "nameIdentifiers": [{"nameIdentifier": "44773", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jian, Zhang"}], "nameIdentifiers": [{"nameIdentifier": "44774", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Keller, Evan T."}], "nameIdentifiers": [{"nameIdentifier": "44775", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Namiki, Mikio"}], "nameIdentifiers": [{"nameIdentifier": "20454", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70155985", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70155985"}, {"nameIdentifier": "70155985", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000070155985"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "HO-PR-MIZOKAMI-A-131.pdf", "filesize": [{"value": "112.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 112000.0, "url": {"label": "HO-PR-MIZOKAMI-A-131.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/26646/files/HO-PR-MIZOKAMI-A-131.pdf"}, "version_id": "ade59e39-20b3-4945-b8e5-6c94f1cb353b"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cDNA microarray", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MDR-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Paclitaxel resistance", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prostate cancer", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The establishment of two paclitaxel resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The establishment of two paclitaxel resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines"}]}, "item_type_id": "4", "owner": "3", "path": ["1763"], "permalink_uri": "http://hdl.handle.net/2297/9569", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-10-05"}, "publish_date": "2017-10-05", "publish_status": "0", "recid": "26646", "relation": {}, "relation_version_is_last": true, "title": ["The establishment of two paclitaxel resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines"], "weko_shared_id": -1}
The establishment of two paclitaxel resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines
http://hdl.handle.net/2297/9569
http://hdl.handle.net/2297/95690b22c934-009a-4b46-bf28-bd1b2dffc564
名前 / ファイル | ライセンス | アクション |
---|---|---|
HO-PR-MIZOKAMI-A-131.pdf (112.0 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | The establishment of two paclitaxel resistant prostate cancer cell lines and the mechanisms of paclitaxel resistance with two cell lines | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takeda, Masashi
× Takeda, Masashi× Mizokami, Atsushi× Mamiya, Kiminori× You, Qiang Li× Jian, Zhang× Keller, Evan T.× Namiki, Mikio |
|||||
提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医学部附属病院泌尿器科 | |||||
書誌情報 |
Prostate 巻 67, 号 9, p. 955-967, 発行日 2007-06-15 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0270-4137 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10621087 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/pros.20581 | |||||
出版者 | ||||||
出版者 | John Wiley & Sons | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | BACKGROUND. Although paclitaxel is used for hormone-resistant prostate cancer, relapse definitely occurs later. Details of the molecular mechanism responsible for paclitaxel- resistance remain unclear. METHODS. We established paclitaxel-resistant cells, DU145-TxR and PC-3-TxR from parent DU145 and PC-3. To characterize these cells, we examined cross-resistance to other anticancer drugs. Expression of several potential genes that had been related to drug-resistance was compared with parent cells by RT-PCR and Western blotting. Methylation analysis of multiple drug resistance (MDR1) promoter was carried out using bisulfite-modified DNA from cell lines. Knockdown experiments using small interfering RNA (siRNA) were also performed to confirm responsibility of drug-resistance. Finally, cDNA microarray was performed to quantify gene expression in PC-3 and PC-3-TxR cells. RESULTS. The IC50 for paclitaxel in DU145-TxR and PC-3-TxR was 34.0- and 43.4-fold higher than that in both parent cells, respectively. Both cells showed cross-resistance to some drugs, but not to VP-16 and cisplatin. Methylation analysis revealed that methylated CpG sites of MDR1 promoter in DU145 and PC-3 cells were demethylated in DU145-TxR cells, but not in PC-3-TxR cells. Knockdown of P-glycoprotein (P-gp), which was up-regulated in resistant cells, by MDR-1 siRNA restored paclitaxel sensitivity in DU145-TxR but not in PC-3-TxR, indicating that upregulation of P-gp was not always main cause of paclitaxel-resistance. Microarray analysis identified 201 (1.34%) up-regulated genes and 218 (1.45%) out of screened genes in PC-3-TxR. CONCLUSIONS. Our data will provide molecular mechanisms of paclitaxel-resistance and be useful for screening target genes to diagnose paclitaxel sensitivity. © 2007 Wiley-Liss, Inc. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |