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Identification of 20-hydroxyecdysone-inducible genes from larval brain of the silkworm, bombyx mori, and their expression analysis
http://hdl.handle.net/2297/31376
http://hdl.handle.net/2297/313769ba5b5bc-1578-4b17-8554-ad3e74df2394
名前 / ファイル | ライセンス | アクション |
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SC-PR-IWAMI-M-333.pdf (219.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Identification of 20-hydroxyecdysone-inducible genes from larval brain of the silkworm, bombyx mori, and their expression analysis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Roy, Anuradha
× Roy, Anuradha× Shimizu, Sakiko× Kiya, Taketoshi× Mita, Kazuei× Iwami, Masafumi |
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書誌情報 |
Zoological Science 巻 29, 号 5, p. 333-339, 発行日 2012-05-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0289-0003 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10545874 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2108/zsj.29.333 | |||||
出版者 | ||||||
出版者 | 日本動物学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The insect brain secretes prothoracicotropic hormone (PTTH), which stimulates the prothoracic gland to synthesize ecdysone. The active metabolite of ecdysone, 20-hydroxyecdysone (20E), works through ecdysone receptor (EcR) and ultraspiracle (USP) to initiate molting and metamorphosis by regulating downstream genes. Previously, we found that EcR was expressed in the PTTH-producing neurosecretory cells (PTPCs) in larval brain of the silkworm Bombyx mori, suggesting that PTPCs function as the master cells of development under the regulation of 20E. To gain a better understanding of the molecular mechanism of the 20E control of PTPCs, we performed a comprehensive screening of genes induced by 20E using DNA microarray with brains of day-2 fifth instar silkworm larvae. Forty-one genes showed greater than twofold changes caused by artificial application of 20E. A subsequent semiquantitative screening identified ten genes upregulated by 20E, four of which were novel or not previously identified as 20E-response genes. Developmental profiling determined that two genes, UP4 and UP5, were correlated with the endogenous ecdysteroid titer. Whole-mount in situ hybridization showed exclusive expression of these two genes in two pairs of cells in the larval brain in response to 20E-induction, suggesting that the cells are PTPCs. BLAST searches revealed that UP4 and UP5 are Bombyx homologs of vrille and tarsal-less, respectively. The present study identifies 20E-induced genes that may be involved in the ecdysone signal hierarchies underlying pupal-adult development and/or the 20E regulation of PTPCs. © 2012 Zoological Society of Japan. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 発行後1年より全文公開 | |||||
権利 | ||||||
権利情報 | © 2012 Zoological Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.zoology.or.jp/ |