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Alpha-glucosidase-like activity detected in a siboglinid polychaete, Oligobrachia mashikoi
http://hdl.handle.net/2297/10950
http://hdl.handle.net/2297/1095032fb2533-8438-4504-a27f-8014b07634b8
名前 / ファイル | ライセンス | アクション |
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SC-PR-KOIZUMI-T-364.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Alpha-glucosidase-like activity detected in a siboglinid polychaete, Oligobrachia mashikoi | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Koizumi, Takashi
× Koizumi, Takashi× Sasayama, Yuichi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 元金沢大学大学院自然科学研究科生命科学専攻 / キッセイ薬品工業株式会社 開発研究部 薬理研究所 | |||||
提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学環日本海域環境研究センター生物多様性研究部門 | |||||
書誌情報 |
Zoological Science 巻 25, 号 4, p. 364-371, 発行日 2008-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0289-0003 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10545874 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2108/zsj.25.364 | |||||
出版者 | ||||||
出版者 | 日本動物学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Siboglinid worms live on carbohydrates produced by symbiotic bacteria. In this study, α-glucosidase-like activity was detected in the surface of the body and in the trophosome of Oligobrachia mashikoi. The enzyme exhibiting this activity was partially purified by consecutively applying the crude enzyme extract to Con-A-Sepharose and Sephadex-200 HR columns. The enzyme sample thus obtained gave a single activity peak at a position corresponding to 550 kDa in the Sephadex-200 HR gel filtration column. The enzyme was active in the range of pH 6.0-8.0, with a maximum activity at around pH 6.5. It specifically hydrolyzed maltose, and was inhibited by voglibose and miglitol. Moreover, a glucose transporter 2-like protein was detected by immunohistochemical and Western-blotting analyses using anti-rat GLUT2 polyclonal antibody. These results raise the question how this unique species lives. © 2008 Zoological Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |