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Investigation of C-3 epimerization mechanism of 24,25-dihydroxyvitamin D3 in rat using liquid chromatography/mass spectrometry
http://hdl.handle.net/2297/29326
http://hdl.handle.net/2297/2932690599b4e-531d-4694-8132-6b982750e40e
名前 / ファイル | ライセンス | アクション |
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PH-PR-HIGASHI-K-477.pdf (59.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-04 | |||||
タイトル | ||||||
タイトル | Investigation of C-3 epimerization mechanism of 24,25-dihydroxyvitamin D3 in rat using liquid chromatography/mass spectrometry | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Higashi, Tatsuya
× Higashi, Tatsuya× Ogasawara, Aki× Shimada, Kazutake |
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書誌情報 |
Analytical Sciences 巻 16, 号 5, p. 477-482, 発行日 2000-05-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0910-6340 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.16.477 | |||||
出版者 | ||||||
出版者 | Japan Society for Analytical Chemistry = 日本分析化学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Studies on C-3 epimerization of 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] were performed. 3-Epi-24,25(OH)2D3 was found to circulate in the blood of rats administered 24,25(OH)2D3, which was confirmed in comparison with a standard sample based on its chromatographic behavior during inclusion high-performance liquid chromatography using γ-cyclodextrin as a mobile phase additive and data obtained from liquid chromatography (LC)/mass spectrometry (MS) and gas chromatography/MS. The C-3 epimerization mechanism was investigated in in vitro experiments using [3α-2H]-24,25(OH)2D3. The disappearance of deuterium was monitored by LC/MS. As a result, it was clarified that the 3-epimer was formed via the 3-oxo-form as an intermediate. This epimerization was also found to be controlled by the enzyme existing in the liver cytosol fraction and to require NAD and NADPH as coenzymes. | |||||
権利 | ||||||
権利情報 | Copyright (c) 2005 by The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://japanlinkcenter.org/JST.JSTAGE/analsci/16.477 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jsac.or.jp/ |