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Rubredoxin from the green sulfur bacterium Chlorobaculum tepidum donates a redox equivalent to the flavodiiron protein in an NAD(P)H dependent manner via ferredoxin-NAD(P)+ oxidoreductase
https://doi.org/10.24517/00060069
https://doi.org/10.24517/00060069120448c7-c368-4ddc-8f43-22addf9611cc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-SEO-D-2021-799.pdf (5.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2021-03-01 | |||||
| タイトル | ||||||
| タイトル | Rubredoxin from the green sulfur bacterium Chlorobaculum tepidum donates a redox equivalent to the flavodiiron protein in an NAD(P)H dependent manner via ferredoxin-NAD(P)+ oxidoreductase | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| ID登録 | ||||||
| ID登録 | 10.24517/00060069 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Ittarat, Wanwipa
× Ittarat, Wanwipa× Sato, Takeshi× Kitashima, Masaharu× Sakurai, Hidehiro× Inoue, Kazuhito× Seo, Daisuke |
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| 著者別表示 |
井上, 和仁
× 井上, 和仁× 瀨尾, 悌介 |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学理工研究域物質化学系 | |||||
| 書誌情報 |
Archives of Microbiology 巻 203, p. 799-808, 発行日 2020-10-14 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0302-8933 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00548209 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s00203-020-02079-4 | |||||
| 出版者 | ||||||
| 出版者 | Springer-Verlag | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The green sulfur bacterium, Chlorobaculum tepidum, is an anaerobic photoautotroph that performs anoxygenic photosynthesis. Although genes encoding rubredoxin (Rd) and a putative flavodiiron protein (FDP) were reported in the genome, a gene encoding putative NADH-Rd oxidoreductase is not identified. In this work, we expressed and purified the recombinant Rd and FDP and confirmed dioxygen reductase activity in the presence of ferredoxin-NAD(P)+ oxidoreductase (FNR). FNR from C. tepidum and Bacillus subtilis catalyzed the reduction of Rd at rates comparable to those reported for NADH-Rd oxidoreductases. Also, we observed substrate inhibition at high concentrations of NADPH similar to that observed with ferredoxins. In the presence of NADPH, B. subtilis FNR and Rd, FDP promoted dioxygen reduction at rates comparable to those reported for other bacterial FDPs. Taken together, our results suggest that Rd and FDP participate in the reduction of dioxygen in C. tepidum and that FNR can promote the reduction of Rd in this bacterium. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | This work was partly supported by Japan Society for the Promotion of Science KAKENHI Grant Number JP17K07304 (to DS) and JP18K06296 (to KI). Acknowledgements | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Embargo Period 12 months | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2020, Springer-Verlag GmbH Germany, part of Springer Nature. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.springer.com/journal/203 | |||||
| 関連名称 | https://www.springer.com/journal/203 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://link.springer.com/article/10.1007/s00203-020-02079-4 | |||||
| 関連名称 | https://link.springer.com/article/10.1007/s00203-020-02079-4 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K07304/ | |||||
| 関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17K07304/ | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K06296/ | |||||
| 関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18K06296/ | |||||
