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Microstructural and Genetic Insights Into the Formation of the “Winter Diffusion Layer” in Japanese Pearl Oyster Pinctada fucata and Its Relation to Environmental Temperature Changes
https://doi.org/10.24517/00067129
https://doi.org/10.24517/0006712994ca16cf-3bc4-4da3-8edd-6b4ac749b2b0
名前 / ファイル | ライセンス | アクション |
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FR-PR-SATO-K-10-794287.pdf (5.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-09-29 | |||||
タイトル | ||||||
タイトル | Microstructural and Genetic Insights Into the Formation of the “Winter Diffusion Layer” in Japanese Pearl Oyster Pinctada fucata and Its Relation to Environmental Temperature Changes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00067129 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sato, Kei
× Sato, Kei× Davin H. E., Setiamarga× Yonemitsu, Hiroshi× Higuchi, Keita |
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著者別表示 |
佐藤, 圭
× 佐藤, 圭 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学国際基幹教育院 GS教育系 | |||||
書誌情報 |
Frontiers in Ecology and Evolution 巻 10, p. 794287, 発行日 2022-03-17 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2296-701X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fevo.2022.794287 | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Phenotypic plasticity in molluscan shell microstructures may be related to environmental changes. The “winter diffusion layer,” a shell microstructure of the Japanese pearl oyster Pinctada fucata, is an example of this phenomenon. In this study, we used P. fucata specimens with shared genetic background to evaluate the seasonal plasticity of shell microstructures, at molecular level. To detect the seasonal changes in shell microstructure and mineral composition, shells of multiple individuals were periodically collected and analyzed using scanning electron microscopy and Raman spectrophotometry. Our observations of the winter diffusion layer revealed that this irregular shell layer, located between the outer and middle shell layers, had a sphenoid shape in radial section. This distinct shape might be caused by the internal extension of the outer shell layer resulting from growth halts. The winter diffusion layer could be distinguished from the calcitic outer shell layer by its aragonitic components and microstructures. Moreover, the components of the winter diffusion layer were irregular simple prismatic (the outer and inner sublayers) and homogeneous structures (the middle sublayer). This irregular formation occurred until April, when the animals resumed their “normal” shell formation after hibernation. To check for a correlation between gene expression and the changes in microstructures, we conducted qPCR of seven major biomineralization-related shell matrix protein-coding genes (aspein, prismalin-14, msi7, msi60, nacrein, n16, and n19) in the shell-forming mantle tissue. Tissue samples were collected from the mantle edge (tissue secreting the outer shell layer) and mantle pallium (where the middle shell layer is constructed) of the same individuals used for microstructural observation and mineral identification that were collected in January (winter growth break period), April (irregular shell formation period), and August (normal shell formation period). Statistically significant differences in gene expression levels were observed between mantle edge and mantle pallium, but no seasonal differences were detected in the seasonal expression patterns of these genes. These results suggest that the formation of the irregular shell layer in P. fucata is caused by a currently unknown genetic mechanism unrelated to the genes targeted in the present study. Further studies using big data (transcriptomics and manipulation of gene expression) are required to answer the questions herein raised. Nevertheless, the results herein presented are essential to unravel the intriguing mystery of the formation of the winter diffusion layer, which may allow us to understand how marine mollusks adapt or acclimate to climate changes. Copyright © 2022 Sato, Setiamarga, Yonemitsu and Higuchi. | |||||
権利 | ||||||
権利情報 | Copyright © 2022 Sato, Setiamarga, Yonemitsu and Higuchi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fevo.2022.794287/full | |||||
関連名称 | https://www.frontiersin.org/articles/10.3389/fevo.2022.794287/full | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://frontiersin.org/Ecology_and_Evolution | |||||
関連名称 | http://frontiersin.org/Ecology_and_Evolution | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.frontiersin.org/ | |||||
関連名称 | http://www.frontiersin.org/ |