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固体NMRによる結晶化が困難なタンパク質のための構造解析法の開発
https://doi.org/10.24517/00051955
https://doi.org/10.24517/000519551035f905-1661-496e-b9aa-5963c5d35cc4
名前 / ファイル | ライセンス | アクション |
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SC-PR-OHASHI-R-kaken 2014-4p.pdf (420.8 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-08-23 | |||||
タイトル | ||||||
タイトル | 固体NMRによる結晶化が困難なタンパク質のための構造解析法の開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of the structure analytical method for protein having difficulty in crystallization by the solid-state NMR | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00051955 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Ohashi, Ryutaro
× Ohashi, Ryutaro |
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書誌情報 |
平成25(2013)年度 科学研究費補助金 若手研究(B) 研究成果報告書 en : 2013 Fiscal Year Final Research Report 巻 2012-04-01 - 2014-03-31, p. 4p., 発行日 2014-06-03 |
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出版者 | ||||||
出版者 | 金沢大学理工研究域物質化学系 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では、固体2次元交換核磁気共鳴測定を用いてアミノ酸の固体粉末試料の炭素核距離を解析した。試料には2つの炭素原子核を炭素13で標識したアミノ酸(ロイシン, アラニン)を用いた。解析の結果、アラニンのメチン基とメチル基の炭素核間距離(文献値は1.53Å)は0.9~1.6Åと見積もることができた。また、ロイシンのメチン基とカルボキシ基の炭素核間距離(文献値は1.53Å)は1.6Å以下と見積もられたが、距離の範囲の下限を見積もることはできなかった。以上より、本手法は水素核が直接結合した炭素同士であれば、距離の範囲を見積もることができる可能性が示唆された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this study, the distances between two carbon nuclei in amino acids were estimated from time-dependence of signals recorded by measurements of two-dimensional exchange solid-state nuclear magnetic resonance. We analyzed two amino acids (l-Leucine, l-Alanine) which were labeled two carbon nuclei with carbon-13. We succeeded to estimate the distance between the two carbons of methine and methyl groups in l-Alanine from 0.9 to 1.6 angstroms. Although it was estimated that the distance between carbon nuclei of methine and carboxyl groups in l-Leucine was less than 1.6 angstroms, we could not estimate the lower limit of the distance of l-Leucine. From the above, it is indicated that ranges of distances among carbon nuclei directly-bonding hydrogen nuclei are possibly estimated. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:24750014, 研究期間(年度):2012-04-01 - 2014-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「固体NMRによる結晶化が困難なタンパク質のための構造解析法の開発」課題番号24750014 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24750014/24750014seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=50533577 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=50533577 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24750014/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24750014/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24750014/24750014seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-24750014/24750014seika/ |