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小腸絨毛上皮モデルを用いた革新的in vitro薬物吸収性予測システムの開発
https://doi.org/10.24517/00058706
https://doi.org/10.24517/0005870615b332cf-8bda-4569-9e8d-43444871984f
名前 / ファイル | ライセンス | アクション |
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PH-PR-SHIRASAKA-Y-kaken 2019-5p.pdf (349.1 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2020-07-17 | |||||
タイトル | ||||||
タイトル | 小腸絨毛上皮モデルを用いた革新的in vitro薬物吸収性予測システムの開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Evaluation of Oral Drug Absorption by Developing In Vitro Culture System for Intestinal Villus Cells | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00058706 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Shirasaka, Yoshiyuki
× Shirasaka, Yoshiyuki |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域薬学系 / 東京薬科大学 | |||||
書誌情報 |
平成30(2018)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書 en : 2018 Fiscal Year Final Research Report 巻 2016-04-01 – 2019-03-31, p. 5p., 発行日 2019-06-13 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 小腸絨毛上皮モデルの構築を目的として、Transwellシステムに流路を作製し、Caco-2細胞の灌流培養を試みた。タイトジャンクション指標となるTEERは、通常の培養条件下に比べ顕著に高い値を示し、灌流培養法による細胞形態変化が示唆された。一方、MUCおよびCYP3A4のmRNA発現に低下傾向が示されたことから、より妥当な細胞モデルの獲得が必要と考えられた。そこでCYP3A4安定発現系の作成を試みた結果、顕著なCYP3A4発現/活性が確認された。本細胞の灌流培養による機能形態変化が期待されることから、今後、小腸絨毛上皮モデルの構築と活用を目指した発展的な研究が期待される。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To develop intestinal villus model, a flow channel was built in the Transwell system, and Caco-2 cells was cultured under steady flow conditions. As a result, this culture method markedly increased TEER, which shows well-formed tight junctions compared to general culture conditions, suggesting an alteration of cellular morphology. On the other hand, mRNA expressions of MUC and CYP3A4 was decreased, and it was considered necessary to obtain alternative cell models. Therefore, next, we tried to establish a CYP3A4 stable expression system. Consequently, remarkable CYP3A4 expression and activity were confirmed in the cells. Change in this cellular morphology is expected by means of cell culture method with steady flow conditions. Furthermore, it is expected that this methodology contributes to improve the prediction efficiency of in vivo intestinal drug absorption. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:16K15165, 研究期間(年度):2016-04-01 – 2019-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「小腸絨毛上皮モデルを用いた革新的in vitro薬物吸収性予測システムの開発」課題番号16K15165 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K15165/16K15165seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/search/?kw=60453833 | |||||
関連名称 | https://kaken.nii.ac.jp/ja/search/?kw=60453833 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K15165/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K15165/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K15165/16K15165seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K15165/16K15165seika/ |