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Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor
http://hdl.handle.net/2297/7443
http://hdl.handle.net/2297/744357ba51c4-6e82-4390-a07c-45fb545e031b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-ANDO-T-379.pdf (195.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Amitani, Ichiro
× Amitani, Ichiro× Sakamoto, Takeshi× Ando, Toshio |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学大学院自然科学研究科物理学 | |||||
| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学理学部 | |||||
| 書誌情報 |
Biophysical Journal 巻 80, 号 1, p. 379-397, 発行日 2001-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-3495 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00566095 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/s0006-3495(01)76022-5 | |||||
| 出版者 | ||||||
| 出版者 | Biophysical Society | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have attempted to link the solution actomyosin ATPase with the mechanical properties of in vitro actin filament sliding over heavy meromyosin. To accomplish this we perturbed the system by altering the substrate with various NTPs and divalent cations, and by altering ionic strength. A wide variety of enzymatic and mechanical measurements were made under very similar solution conditions. Excellent correlations between the mechanical and enzymatic quantities were revealed. Analysis of these correlations based on a force-balance model led us to two fundamental equations, which can be described approximately as follows: the maximum sliding velocity is proportional to √VmaxKm/A, where Km/A is the actin concentration at which the substrate turnover rate is half of its maximum (Vmax). The active force generated by a cross-bridge under no external load or under a small external load is proportional to √Vmax/Km/A. The equations successfully accounted for the correlations observed in the present study and observations in other laboratories. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
