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Analysis of magnetotactic behavior by swimming assay
https://doi.org/10.24517/00010487
https://doi.org/10.24517/00010487509e1378-4ed6-408d-ac3c-165e17123271
| 名前 / ファイル | ライセンス | アクション |
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SC-PR-FUKUMORI-Y-940.pdf (433.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2017-10-03 | |||||||||
| タイトル | ||||||||||
| タイトル | Analysis of magnetotactic behavior by swimming assay | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.24517/00010487 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Sakaguchi, Shoutaro
× Sakaguchi, Shoutaro× Taoka, Azuma× Fukumori, Yoshihiro |
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| 著者別表示 |
田岡 , 東
× 田岡 , 東
× 福森, 義宏
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| 書誌情報 |
Bioscience, Biotechnology and Biochemistry 巻 77, 号 5, p. 940-947, 発行日 2013-01-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0916-8451 | |||||||||
| NCID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA10824164 | |||||||||
| DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1271/bbb.120880 | |||||||||
| 出版者 | ||||||||||
| 出版者 | 日本農芸化学会 = Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Prokaryotic organelles called magnetosomes allow magnetotactic bacteria to navigate along geomagnetic field lines. In this study, we modified a swimming assay commonly used to assess bacterial motility to develop a new method of assessing magnetotactic motility. By this method, the swimming assay was performed in an artificial magnetic field. Magnetotactic bacteria formed a wedge-shaped swimming halo that elongated parallel to the magnetic field. Magnetotactic motility was qualitatively assessed by comparing halo shapes. We termed this method the magnetic swimming assay. On the magnetic swimming assay, the mamK deletion strain formed a shorter halo than the wild type, indicating that the assay sensitively detects differences in magnetotactic motility. Moreover, we isolated two spontaneous magnetotactic motility mutants using magnetic swimming plates. Our findings indicate that the magnetic swimming assay is a useful method for the sensitive analysis of magnetotaxis phenotypes and mutant screening. | |||||||||
| 権利 | ||||||||||
| 権利情報 | Copyright © Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | http://www.jsbba.or.jp/ | |||||||||
| 関連URI | ||||||||||
| 識別子タイプ | URI | |||||||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/bbb | |||||||||
