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Solubilization of the ecdysone binding protein from anterior silk gland cell membranes of the silkworm, bombyx mori
http://hdl.handle.net/2297/28990
http://hdl.handle.net/2297/28990d19c366f-ee21-4785-8f9d-cd8d617c1f80
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-SAKURAI-S-971.pdf (340.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Solubilization of the ecdysone binding protein from anterior silk gland cell membranes of the silkworm, bombyx mori | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Elmogy, Mohamed
× Elmogy, Mohamed× Iwami, Masafumi× Sakurai, Sho |
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| 書誌情報 |
Zoological science 巻 24, 号 10, p. 971-977, 発行日 2007-10-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0289-1824 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2108/zsj.24.971 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan = 日本動物学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We previously provided preliminary evidence for the presence of a putative membrane ecdysone receptor (mEcR) anchored in the plasma membranes of anterior silk glands (ASGs) in Bombyx mori. This receptor may act in concert with the conventional EcR in 20E-dependent programmed cell death of these glands. We report here, for the first time, the solubilization of mEcR from ASG membranes using the zwitterionic detergent CHAPS in the presence of NaCl. Our results show by ligand binding assay that mEcR solubilized this way is functionally active and retains 75% of its native binding activity. We also defined experimental conditions that yielded protein/detergent complexes with partial binding activity, which makes it possible to purify the membrane-bound ecdysone binding protein. © 2007 Zoological Society of Japan. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.zoology.or.jp/ | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.bioone.org/doi/abs/10.2108/zsj.24.971 | |||||
