| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
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|
タイトル |
Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00010925 |
|
ID登録タイプ |
JaLC |
| 著者 |
Yamamoto, Daisuke
Taoka, Azuma
Uchihashi, Takayuki
Sasaki, Hideaki
Watanabe, Hiroki
Ando, Toshio
Fukumori, Yoshihiro
|
| 著者別表示 |
山本, 大輔
田岡 , 東
内橋, 貴之
安藤, 敏夫
福森, 義宏
|
| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学理工研究域数物科学系 |
| 書誌情報 |
Proceedings of the National Academy of Sciences
巻 107,
号 20,
p. 9382-9387,
発行日 2010-05-18
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0027-8424 |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10808769 |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1073/pnas.1001870107 |
| 出版者 |
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出版者 |
National Academy of Sciences (U.S.) |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The unique ability of magnetotactic bacteria to navigate along a geomagnetic field is accomplished with the help of prokaryotic organelles, magnetosomes. The magnetosomes have well-ordered chain-like structures, comprising membrane-enveloped, nano-sized magnetic crystals, and various types of specifically associated proteins. In this study, we applied atomic force microscopy (AFM) to investigate the spatial configuration of isolated magnetosomes from AMB-1 in near-native buffer conditions. AFM observation revealed organic material with a ∼7-nm thickness surrounding a magnetite crystal. Small globular proteins, identified as magnetosome-associated protein MamA, were distributed on the mica surface around the magnetosome. Immuno-labeling with AFM showed that MamA is located on the magnetosome surface. In vitro experiments showed that MamA proteins interact with each other and form a high molecular mass complex. These findings suggest that magnetosomes are covered with MamA oligomers in near-native environments. Furthermore, nanodissection revealed that magnetosomes are built with heterogeneous structures that comprise the organic layer. This study provides important clues to the supramolecular architecture of the bacterial organelle, the magnetosome, and insight into the function of the proteins localized in the organelle. |
| 著者版フラグ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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|
識別子タイプ |
URI |
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|
関連識別子 |
http://www.pnas.org/content/early/2010/04/30/1001870107.abstract |
SC-PR-YAMAMOTO-D-2010.pdf (1.4 MB)
