WEKO3
アイテム
Visualization of the specific gene transcription in the nucleus with a novel in situ RNase protection method
http://hdl.handle.net/2297/6774
http://hdl.handle.net/2297/6774365b8f44-0579-4207-b7ba-8e99bf9c42fb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA00508022-WAKAYAMA-T-305.pdf (916.1 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Visualization of the specific gene transcription in the nucleus with a novel in situ RNase protection method | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
井関, 尚一
× 井関, 尚一× 若山, 友彦 |
|||||
| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学大学院医学系研究科がん細胞学 | |||||
| 書誌情報 |
Acta Histochemica et Cytochemica 巻 33, 号 4, p. 305-313, 発行日 2000-01-01 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0044-5991 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508022 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1267/ahc.33.305 | |||||
| 出版者 | ||||||
| 出版者 | 日本組織細胞化学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The ordinary in situ hybridization technique, using the labeled DNA or RNA probes, visualizes the mRNA species that have previously been transcribed and accumulated in the cytoplasm. In contrast, we aimed to visualize the transcription of particular genes itself by use of the hybridization between radioisotope-labeled newly transcribed cellular RNA and unlabeled RNA probes (riboprobes) followed by digestion of unhybridized RNA with RNase. In the present study, the cultured mouse 3T3-L1 cell line with or without the induction for the adipocyte was labeled with 3H-uridine, fixed and was hybridized in situ with the complementary (antisense) or homologous (sense) riboprobe for 28S rRNA or glycerol-3-phosphate dehydrogenase (GPDH) mRNA, an adipocyte marker. Following extensive digestion with RNase, the signal for remaining radioactivity in nuclei was quantitatively analyzed with autoradiography. In the uninduced cells hybridized with 28S rRNA probes, a significantly stronger signal was obtained with the complementary probe than with the homologous probe. With respect to GPDH probes, the adipocyte-induced cells showed a significantly stronger signal with the complementary probe than with the homologous probe, whereas the uninduced cells showed no significant difference in signal with both probes. These results demonstrated the possibility of visualizing the transcription of particular genes in particular cells at particular time point by applying 'in situ RNase protection' to histological specimens. | |||||
| 権利 | ||||||
| 権利情報 | 日本組織細胞化学会の許諾を得て登録 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/ahc/33/4/33_305/_article/-char/en | |||||
