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Detection of cfxA and cfxA2, the β-lactamase genes of Prevotella spp., in clinical samples from dentoalveolar infection by real-time PCR
http://hdl.handle.net/2297/1824
http://hdl.handle.net/2297/18244b373e63-9174-4dca-9541-b6c382a83f5d
| 名前 / ファイル | ライセンス | アクション |
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ME-PR-KARASAWA-cfx revised submitted.pdf (113.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Detection of cfxA and cfxA2, the β-lactamase genes of Prevotella spp., in clinical samples from dentoalveolar infection by real-time PCR | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Iwahara, Kaori
× Iwahara, Kaori× Kuriyama, Tomoari× Shimura, Satoshi× Williams, David W.× Yanagisawa, Maki× Nakagawa, Kiyomasa× Karasawa, Tadahiro |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学大学院医学系研究科 | |||||
| 書誌情報 |
Journal of clinical microbiology 巻 44, 号 1, p. 172-176, 発行日 2006-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0095-1137 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1128/jcm.44.1.172-176.2006 | |||||
| 出版者 | ||||||
| 出版者 | American Society for Microbiology | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | While most bacteria involved in dentoalveolar infection are highly susceptible to penicillin, some Prevotella strains exhibit resistance to this agent through the production of ß-lactamase. The production of ß-lactamase by Prevotella spp. is in turn associated with the expression of the genes cfxA and cfxA2. The aim of the present study was to determine the prevalence of cfxA and cfxA2 in Prevotella strains by use of real-time PCR and to assess the performance of this molecular method for the direct detection of the genes in 87 clinical samples (pus and root canal exudates) from dentoalveolar infection. Production of ß-lactamase by each isolate was determined using a nitrocefin disk. ß-Lactamase production was seen in 31% of Prevotella isolates, while all isolates of other species were ß-lactamase negative. The penicillin resistance of isolates strongly correlated with the production of ß-lactamase. Real-time PCR was found to detect the cfxA and cfxA2 genes from at least five cells per reaction mixture (5 x 103 CFU/ml of pus). Using real-time PCR, the presence of cfxA and cfxA2 was evident for all 48 ß-lactamase-positive Prevotella strains. In contrast, neither ß-lactamase-negative Prevotella (n = 91) or non-Prevotella (n = 31) strains were positive for the genes. In this study, 31 of the 87 samples yielded ß-lactamase-positive Prevotella results, and cfxA and cfxA2 were detected in all 31 samples. Of the 56 culture-negative samples, 8 (14%) were positive for cfxA and cfxA2 by the real-time PCR. This sensitive and specific molecular method offers a rapid clinical test for aiding in the selection of an appropriate antibiotic for treatment of dentoalveolar infection. Although penicillin remains largely effective in the treatment of dentoalveolar infection, ß-lactamase-stable antibiotics should be considered in cases in which ß-lactamase-positive Prevotella strains are involved. | |||||
| 権利 | ||||||
| 権利情報 | copyright©American Society for Microbiology, Journal of clinical microbiology, volume44(1), 172-176, 2006 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
