| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
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タイトル |
Upregulation of Leukemia Inhibitory Factor (LIF) during the Early Stage of Optic Nerve Regeneration in Zebrafish. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00014057 |
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ID登録タイプ |
JaLC |
| 著者 |
Ogai, Kazuhiro
Kuwana, Ayaka
Hisano, Suguru
Nagashima, Mikiko
Koriyama, Yoshiki
Sugitani, Kayo
Mawatari, Kazuhiro
Nakashima, Hiroshi
Kato, Satoru
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| 著者別表示 |
大貝, 和裕
郡山, 恵樹
杉谷, 加代
馬渡, 一浩
中島, 広志
加藤, 聖
|
| 書誌情報 |
PLOS ONE
巻 9,
号 8,
p. e106010,
発行日 2014-08-27
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| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1371/journal.pone.0106010 |
| 出版者 |
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出版者 |
PLOS |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Fish retinal ganglion cells (RGCs) can regenerate their axons after optic nerve injury, whereas mammalian RGCs normally fail to do so. Interleukin 6 (IL-6)-type cytokines are involved in cell differentiation, proliferation, survival, and axon regrowth; thus, they may play a role in the regeneration of zebrafish RGCs after injury. In this study, we assessed the expression of IL-6-type cytokines and found that one of them, leukemia inhibitory factor (LIF), is upregulated in zebrafish RGCs at 3 days post-injury (dpi). We then demonstrated the activation of signal transducer and activator of transcription 3 (STAT3), a downstream target of LIF, at 3–5 dpi. To determine the function of LIF, we performed a LIF knockdown experiment using LIF-specific antisense morpholino oligonucleotides (LIF MOs). LIF MOs, which were introduced into zebrafish RGCs via a severed optic nerve, reduced the expression of LIF and abrogated the activation of STAT3 in RGCs after injury. These results suggest that upregulated LIF drives Janus kinase (Jak)/STAT3 signaling in zebrafish RGCs after nerve injury. In addition, the LIF knockdown impaired axon sprouting in retinal explant culture in vitro; reduced the expression of a regeneration-associated molecule, growth-associated protein 43 (GAP-43); and delayed functional recovery after optic nerve injury in vivo. In this study, we comprehensively demonstrate the beneficial role of LIF in optic nerve regeneration and functional recovery in adult zebrafish. |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0106010 |
ME-PR-KATO-S-0106010.pdf (16.2 MB)
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