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Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte
http://hdl.handle.net/2297/17052
http://hdl.handle.net/2297/17052ff77958a-8867-426d-9214-99b28651e875
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-KAMBAAYASHI-Y-46.pdf (371.8 kB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kambayashi, Yasuhiro
× Kambayashi, Yasuhiro× Thanh Binh, Nguyen× Asakura, Hiroki W.× Hibino, Yuri× Hitomi, Yoshiaki× Nakamura, Hiroyuki× Ogino, Keiki |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||
| 書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 44, 号 1, p. 46-51, 発行日 2009-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0912-0009 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10710201 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3164/jcbn.08-162 | |||||
| 出版者 | ||||||
| 出版者 | 日本酸化ストレス学会 = SFRR Japan | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the present study, we tried to establish an efficient assay for total antioxidant capacity (TAC) in human plasma using a 96-well microplate. TAC was assessed using lag time by antioxidants against the myoglobin-induced oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) with hydrogen peroxide, and expressed as Trolox equivalent. The linearity of the calibration curve with Trolox was maintained with the Trolox concentration range from 2.5 μM to 25 μM (R2 = 0.997). The assay was applied to the measurement of TAC in healthy human plasma. Coefficient of variation in intraday assay was 2.4%. Difference was not observed in interday assay. Plasma TAC of men ((569 ± 41) μM Trolox equivalent; n = 6) was higher than that of women ((430 ± 28) μM Trolox equivalent; n = 4). After the vegetable juice was drunk for 1 week, the increase in plasma TAC was observed in almost all the volunteers. In summary, we developed the efficient assay for plasma TAC using a 96-well microplate. | |||||
| 権利 | ||||||
| 権利情報 | Copyright (c) 2008 by The Editorial Secretariat of JCBN | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jcbn/44/1/44_46/_article | |||||
