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A time- and cost-saving method of producing rat polyclonal antibodies
http://hdl.handle.net/2297/19338
http://hdl.handle.net/2297/193385a498029-bff0-40fe-b57a-d8a94baf3251
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-WAKAYAMA-T-79.pdf (532.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | A time- and cost-saving method of producing rat polyclonal antibodies | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Wakayama, Tomohiko
× Wakayama, Tomohiko× Kato, Yukio× Utsumi, Rie× Tsuji, Akira× Iseki, Shoichi |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学医薬保健研究域 医学系 | |||||
| 書誌情報 |
Acta Histochemica et Cytochemica 巻 39, 号 3, p. 79-87, 発行日 2006-07-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0044-5991 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508022 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1267/ahc.06003 | |||||
| 出版者 | ||||||
| 出版者 | Japan Society of Histochemistry and Cytochemistry = 日本組織細胞化学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Producing antibodies usually takes more than three months. In the present study, we introduce a faster way of producing polyclonal antibodies based on preparation of the recombinant oligopeptide as antigen followed by immunization of rats. Using this method, we produced antisera against two mouse proteins: ERGIC-53 and c-Kit. An expression vector ligated with a pair of complementary synthetic oligodeoxyribonucleotides encoding the protein was introduced into bacteria, and the recombinant oligopeptide fused with the carrier protein glutathione-S-transferase was purified. Wistar rats were immunized by injecting the emulsified antigen subcutaneously into the hind footpads, followed by a booster injection after 2 weeks. One week after the booster, the sera were collected and examined for the antibody titer by immunohistochemistry. Antisera with 1600-fold titer at the maximum were obtained for both antigens and confirmed for their specificity by Western blotting. Anti-ERGIC-53 antisera recognized acinar cells in the sublingual gland, and anti-c-Kit antisera recognized spermatogenic and Leydig cells in the testis. These antisera were applicable to fluorescent double immunostaining with mouse monoclonal or rabbit polyclonal antibodies. Consequently, this method enabled us to produce specific rat polyclonal antisera available for immunohistochemistry in less than one month at a relatively low cost. © 2006 The Japan Society of Histochemistry and Cytochemistry. | |||||
| 権利 | ||||||
| 権利情報 | Copyright (c) 2006 By the Japan Society of Histochemistry and Cytochemistry / 日本組織細胞化学会の許諾を得て登録 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/ahc/39/3/39_79/_article | |||||
