| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-04 |
| タイトル |
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|
タイトル |
Aryl hydrocarbon receptor nuclear translocator in human liver is regulated by miR-24 |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00014983 |
|
ID登録タイプ |
JaLC |
| 著者 |
Oda, Yuki
Nakajima, Miki
Mohri, Takuya
Takamiya, Masataka
Aoki, Yasuhiro
Fukami, Tatsuki
Yokoi, Tsuyoshi
|
| 著者別表示 |
中嶋, 美紀
深見, 達基
横井, 毅
|
| 提供者所属 |
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|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系 |
| 書誌情報 |
Toxicology and Applied Pharmacology
巻 260,
号 3,
p. 222-231,
発行日 2012-05-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0041-008X |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00864435 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.taap.2012.02.012 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Aryl hydrocarbon receptor nuclear translocator (ARNT) forms a heterodimer with aryl hydrocarbon receptor or hypoxia inducible factor 1α to mediate biological responses to xenobiotic exposure and hypoxia. Although the regulation mechanism of the ARNT expression is largely unknown, earlier studies reported that the human ARNT protein level was decreased by hydrogen peroxide or reactive oxygen species. These stimuli increase the miR-24 level in various human cell lines. In silico analysis predicts that some microRNAs including miR-16 and miR-23b may bind to ARNT mRNA. This background prompted us to investigate whether human ARNT is regulated by microRNAs. Overexpression of miR-24 into HuH-7 and HepG2 cells significantly decreased the ARNT protein level, but not the ARNT mRNA level, indicating translational repression. However, overexpression of miR-16 or miR-23b caused no change in the ARNT expression. The miR-24-dependent down-regulation of ARNT decreased the expression of its downstream genes such as CYP1A1 and carbonic anhydrase IX. Luciferase assay was performed to determine the element on the ARNT mRNA to which miR-24 binds. Finally, it was demonstrated that the miR-24 levels in a panel of 26 human livers were inversely correlated with the protein levels or the translational efficiency of ARNT. Taken together, we found that miR-24 negatively regulates ARNT expression in human liver, affecting the expression of its downstream genes. miR-24 would be one of the factors underlying the mechanisms by which ARNT protein is decreased by reactive oxygen species. © 2012 Elsevier Inc. |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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|
識別子タイプ |
URI |
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|
関連識別子 |
http://www.elsevier.com/locate/issn/0041008X |
PH-PR-NAKAJIMA-M-222.pdf (2.5 MB)
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