{"_buckets": {"deposit": "4add2991-4a78-49d9-ba02-d61b5b36cf68"}, "_deposit": {"created_by": 3, "id": "14997", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "14997"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00014997", "sets": ["1135"]}, "author_link": ["26962", "26963", "246", "100149", "34"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-01-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageStart": "e30456", "bibliographicVolumeNumber": "7", "bibliographic_titles": [{"bibliographic_title": "PLoS ONE"}]}]}, "item_4_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "中西, 猛夫"}], "nameIdentifiers": [{"nameIdentifier": "246", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=30541742"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30541742"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000030541742"}]}, {"creatorNames": [{"creatorName": "玉井, 郁巳"}], "nameIdentifiers": [{"nameIdentifier": "100149", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20155237", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20155237"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Urinary excretion accounts for two-thirds of total elimination of uric acid and the remainder is excreted in feces. However, the mechanism of extra-renal elimination is poorly understood. In the present study, we aimed to clarify the mechanism and the extent of elimination of uric acid through liver and intestine using oxonate-treated rats and Caco-2 cells as a model of human intestinal epithelium. In oxonate-treated rats, significant amounts of externally administered and endogenous uric acid were recovered in the intestinal lumen, while biliary excretion was minimal. Accordingly, direct intestinal secretion was thought to be a substantial contributor to extra-renal elimination of uric acid. Since human efflux transporter BCRP/ABCG2 accepts uric acid as a substrate and genetic polymorphism causing a decrease of BCRP activity is known to be associated with hyperuricemia and gout, the contribution of rBcrp to intestinal secretion was examined. rBcrp was confirmed to transport uric acid in a membrane vesicle study, and intestinal regional differences of expression of rBcrp mRNA were well correlated with uric acid secretory activity into the intestinal lumen. Bcrp1 knockout mice exhibited significantly decreased intestinal secretion and an increased plasma concentration of uric acid. Furthermore, a Bcrp inhibitor, elacridar, caused a decrease of intestinal secretion of uric acid. In Caco-2 cells, uric acid showed a polarized flux from the basolateral to apical side, and this flux was almost abolished in the presence of elacridar. These results demonstrate that BCRP contributes at least in part to the intestinal excretion of uric acid as extra-renal elimination pathway in humans and rats. © 2012 Hosomi et al.", "subitem_description_type": "Abstract"}]}, "item_4_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00014984", "subitem_identifier_reg_type": "JaLC"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1371/journal.pone.0030456", "subitem_relation_type_select": "DOI"}}]}, "item_4_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hosomi, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "26962", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Takeo"}], "nameIdentifiers": [{"nameIdentifier": "246", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=30541742"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30541742"}, {"nameIdentifier": "30541742", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000030541742"}]}, {"creatorNames": [{"creatorName": "Fujita, Takuya"}], "nameIdentifiers": [{"nameIdentifier": "26963", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tamai, Ikumi"}], "nameIdentifiers": [{"nameIdentifier": "34", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20155237", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20155237"}, {"nameIdentifier": "20155237", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=20155237"}, {"nameIdentifier": "20155237", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000020155237"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PH-PR-TAMAI-I-e30456.pdf", "filesize": [{"value": "250.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 250400.0, "url": {"label": "PH-PR-TAMAI-I-e30456.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/14997/files/PH-PR-TAMAI-I-e30456.pdf"}, "version_id": "1a924322-61d3-4687-a9fa-ef248b8f8bc8"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2"}]}, "item_type_id": "4", "owner": "3", "path": ["1135"], "permalink_uri": "https://doi.org/10.24517/00014984", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-10-04"}, "publish_date": "2017-10-04", "publish_status": "0", "recid": "14997", "relation": {}, "relation_version_is_last": true, "title": ["Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2"], "weko_shared_id": 3}