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Inhibition of phagocytic killing of Escherichia coli in Drosophila Hemocytes by RNA Chaperone Hfq
http://hdl.handle.net/2297/46175
http://hdl.handle.net/2297/461754a2a7456-53dd-4a0d-9d16-4cbd8d8b01e2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME-PR-NAKANISHI-Y-1298.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-04 | |||||
| タイトル | ||||||
| タイトル | Inhibition of phagocytic killing of Escherichia coli in Drosophila Hemocytes by RNA Chaperone Hfq | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Shiratsuchi, Akiko
× Shiratsuchi, Akiko× Nitta, Mao× Kuroda, Ayumi× Komiyama, Chiharu× Gawasawa, Mitsuko× Shimamoto, Naoto× Tuan, Tran Quoc× Morita, Teppei× Aiba, Hiroji× Nakanishi, Yoshinobu |
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| 書誌情報 |
Journal of Immunology 巻 197, 号 4, p. 1298-1307, 発行日 2016-08-15 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-1767 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00699656 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.4049/jimmunol.1501953 | |||||
| 出版者 | ||||||
| 出版者 | American Association of Immunologists | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An RNA chaperone of Escherichia coli, called host factor required for phage Qb RNA replication (Hfq), forms a complex with small noncoding RNAs to facilitate their binding to target mRNA for the alteration of translation efficiency and stability. Although the role of Hfq in the virulence and drug resistance of bacteria has been suggested, how this RNA chaperone controls the infectious state remains unknown. In the present study, we addressed this issue using Drosophila melanogaster as a host for bacterial infection. In an assay for abdominal infection using adult flies, an E. coli strain with mutation in hfq was eliminated earlier, whereas flies survived longer compared with infection with a parental strain. The same was true with flies deficient in humoral responses, but the mutant phenotypes were not observed when a fly line with impaired hemocyte phagocytosis was infected. The results from an assay for phagocytosis in vitro revealed that Hfq inhibits the killing of E. coli by Drosophila phagocytes after engulfment. Furthermore, Hfq seemed to exert this action partly through enhancing the expression of s38, a stress-responsive s factor that was previously shown to be involved in the inhibition of phagocytic killing of E. coli, by a posttranscriptional mechanism. Our study indicates that the RNA chaperone Hfq contributes to the persistent infection of E. coli by maintaining the expression of bacterial genes, including one coding for s38, that help bacteria evade host immunity. ©2016 by The American Association of Immunologists, Inc. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Embargo Period 12 months | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
