WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "2a46f71d-1c70-45d7-a1ff-2f43421db11d"}, "_deposit": {"created_by": 3, "id": "26905", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "26905"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00026905", "sets": ["1763"]}, "author_link": ["45467", "45468", "458", "1575", "45466", "1590"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-06-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "354", "bibliographicPageStart": "349", "bibliographicVolumeNumber": "38", "bibliographic_titles": [{"bibliographic_title": "Journal of Toxicological Sciences"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Effects of the CYP3A4 intron 6 C\u003eT (CYP3A4*22) polymorphism, which has recently been reported to have a critical role in vivo, were investigated by measuring CYP3A4 protein expression levels and CYP3A4-dependent drug oxidation activities in individual human liver microsomes in vitro. Prior to protein analysis, analysis of DNA samples indicated that 36 Caucasian subjects were genotyped as CYP3A4*1/*1 and five subjects were CYP3A4*1/*22, with a CYP3A4*22 allelic frequency of 6.1%. No CYP3A4*22 alleles were found in the Japanese samples (106 alleles). Individual differences in CYP2D6-dependent dextromethorphan O-demethylation activities in liver microsomes from Caucasians were not affected by either the CYP3A4*1/*22 or CYP3A5*1/*3 genotype. Liver microsomes genotyped as CYP3A4*1/*22 (n = 4) showed significantly lower CYP3A-dependent dextromethorphan N-demethylation, midazolam 1′-hydroxylation, and testosterone 6β-hydroxylation activities, as well as lower expression levels of CYP3A protein (28% of control), compared with those of the CYP3A4*1/*1 group (n = 19). The other polymorphism, CYP3A5*1/*3, did not show these differences (n = 4). The CYP3A4*22 polymorphism was associated with reduced CYP3A4 protein expression levels and resulted in decreased CYP3A4-dependent activities in human livers. The present results suggest an important role of low expression of CYP3A4 protein associated with the CYP3A4*22 allele in the individual differences in drug clearance.", "subitem_description_type": "Abstract"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本毒性学会 = Japanese Society of Toxicological Sciences"}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.2131/jts.38.349", "subitem_relation_type_select": "DOI"}}]}, "item_4_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.jstage.jst.go.jp/browse/jts", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.jsot.jp/", "subitem_relation_type_select": "URI"}}]}, "item_4_rights_23": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright © 2013 The Japanese Society of Toxicology 日本毒性学会"}]}, "item_4_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0388-1350", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "10.2131/jts.38.349", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Okubo, Maho"}], "nameIdentifiers": [{"nameIdentifier": "45466", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murayama, Norie"}], "nameIdentifiers": [{"nameIdentifier": "1575", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90219949", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=90219949"}, {"nameIdentifier": "90219949", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000090219949"}]}, {"creatorNames": [{"creatorName": "Shimizu, Makiko"}], "nameIdentifiers": [{"nameIdentifier": "1590", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90307075", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=90307075"}, {"nameIdentifier": "90307075", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000090307075"}]}, {"creatorNames": [{"creatorName": "Shimada, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "458", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90409384", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=90409384"}, {"nameIdentifier": "90409384", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000090409384"}]}, {"creatorNames": [{"creatorName": "Guengerich, F. Peter"}], "nameIdentifiers": [{"nameIdentifier": "45467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamazaki, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "45468", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "30191274", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000030191274"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PH-PR-SHIMADA-T-349.pdf", "filesize": [{"value": "479.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 479000.0, "url": {"label": "PH-PR-SHIMADA-T-349.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/26905/files/PH-PR-SHIMADA-T-349.pdf"}, "version_id": "a24d645f-ce07-4f4c-9eb4-d4d803905d51"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "P450 3A4", "subitem_subject_scheme": "Other"}, {"subitem_subject": "P450 3A5", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein expression", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ethnic difference", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Impaired polymorphism", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The CYP3A4 intron 6 C\u003eT polymorphism (CYP3A4*22) is associated with reduced CYP3A4 protein level and function in human liver microsomes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The CYP3A4 intron 6 C\u003eT polymorphism (CYP3A4*22) is associated with reduced CYP3A4 protein level and function in human liver microsomes"}]}, "item_type_id": "4", "owner": "3", "path": ["1763"], "permalink_uri": "http://hdl.handle.net/2297/39142", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-10-05"}, "publish_date": "2017-10-05", "publish_status": "0", "recid": "26905", "relation": {}, "relation_version_is_last": true, "title": ["The CYP3A4 intron 6 C\u003eT polymorphism (CYP3A4*22) is associated with reduced CYP3A4 protein level and function in human liver microsomes"], "weko_shared_id": 3}
The CYP3A4 intron 6 C>T polymorphism (CYP3A4*22) is associated with reduced CYP3A4 protein level and function in human liver microsomes
http://hdl.handle.net/2297/39142
http://hdl.handle.net/2297/39142a548dd8f-0559-4b7f-bfbc-31ea0647826b
名前 / ファイル | ライセンス | アクション |
---|---|---|
PH-PR-SHIMADA-T-349.pdf (479.0 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | The CYP3A4 intron 6 C>T polymorphism (CYP3A4*22) is associated with reduced CYP3A4 protein level and function in human liver microsomes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Okubo, Maho
× Okubo, Maho× Murayama, Norie× Shimizu, Makiko× Shimada, Tsutomu× Guengerich, F. Peter× Yamazaki, Hiroshi |
|||||
書誌情報 |
Journal of Toxicological Sciences 巻 38, 号 3, p. 349-354, 発行日 2013-06-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0388-1350 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10.2131/jts.38.349 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.2131/jts.38.349 | |||||
出版者 | ||||||
出版者 | 日本毒性学会 = Japanese Society of Toxicological Sciences | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Effects of the CYP3A4 intron 6 C>T (CYP3A4*22) polymorphism, which has recently been reported to have a critical role in vivo, were investigated by measuring CYP3A4 protein expression levels and CYP3A4-dependent drug oxidation activities in individual human liver microsomes in vitro. Prior to protein analysis, analysis of DNA samples indicated that 36 Caucasian subjects were genotyped as CYP3A4*1/*1 and five subjects were CYP3A4*1/*22, with a CYP3A4*22 allelic frequency of 6.1%. No CYP3A4*22 alleles were found in the Japanese samples (106 alleles). Individual differences in CYP2D6-dependent dextromethorphan O-demethylation activities in liver microsomes from Caucasians were not affected by either the CYP3A4*1/*22 or CYP3A5*1/*3 genotype. Liver microsomes genotyped as CYP3A4*1/*22 (n = 4) showed significantly lower CYP3A-dependent dextromethorphan N-demethylation, midazolam 1′-hydroxylation, and testosterone 6β-hydroxylation activities, as well as lower expression levels of CYP3A protein (28% of control), compared with those of the CYP3A4*1/*1 group (n = 19). The other polymorphism, CYP3A5*1/*3, did not show these differences (n = 4). The CYP3A4*22 polymorphism was associated with reduced CYP3A4 protein expression levels and resulted in decreased CYP3A4-dependent activities in human livers. The present results suggest an important role of low expression of CYP3A4 protein associated with the CYP3A4*22 allele in the individual differences in drug clearance. | |||||
権利 | ||||||
権利情報 | Copyright © 2013 The Japanese Society of Toxicology 日本毒性学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/jts | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jsot.jp/ |