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Disintegration of lysosomes mediated by GTP γS-treated cytosol: Possible involvement of phospholipases
https://doi.org/10.24517/00026996
https://doi.org/10.24517/00026996337a0704-3ddf-4eaa-ba07-b3d11fa4c3c4
| 名前 / ファイル | ライセンス | アクション |
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PH-PR-SAI-Y-630.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2017-10-05 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Disintegration of lysosomes mediated by GTP γS-treated cytosol: Possible involvement of phospholipases | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24517/00026996 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
Sai, Yoshimichi
× Sai, Yoshimichi× Matsuda, Tomoko× Arai, Kunizo× Ohkuma, Shoji |
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| 著者別表示 |
崔, 吉道
× 崔, 吉道
× 荒井, 國三
× 大熊, 勝治
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| 提供者所属 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 金沢大学附属病院薬剤部 | |||||||||||
| 書誌情報 |
Journal of Biochemistry 巻 123, 号 4, p. 630-636, 発行日 1998-04-01 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0021-924X | |||||||||||
| NCID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00694073 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1093/oxfordjournals.jbchem.a021984 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 日本生化学会 = Japanese Biochemical Society | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | We showed previously that cytosol treated with guanosine 5'-O-(3-thiotriphosphate) (GTP-γS) disintegrated lysosomes in vitro in time-, temperature-, and dose-dependent manners. This also requires ATP, however, the latter can be substituted with deoxy-ATP, ADP, or ATPγS, suggesting no requirement of ATP hydrolysis. The lysis was inhibited by several chemical modifiers, including N-ethylmaleimide, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid, and by various phospholipase inhibitors (trifluoperazine, p-bromophenacyl bromide, nordihydroguaiaretic acid, W-7, primaquine, compound 48/80, neomycin, and gentamicin), but not by ONO-RS-082, an inhibitor of phospholipase A2. The reaction was also inhibited by phospholipids (phosphatidylinositol, phosphatidylserine, phosphatidic acid, and phosphatidylcholine) and diacylglycerol. Among the phospholipase A2 hydrolysis products of phospholipids, unsaturated fatty acids (oleate, linoleate, and arachidonate) and lysophospholipid (lysophosphatidylcholine) by themselves broke lysosomes down directly, whereas saturated fatty acids (palmitate and stearate) had little effect. We found that GTPγS-stimulated cytosolic phospholipase A2 activity was highly sensitive to ONO-RS-082. These results suggest the participation of phospholipase(s), though not cytosolic phospholipase A2, in the GTPγS-dependent lysis of lysosomes. | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | Copyright(c) 2005 by the Japanese Biochemical Society | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 関連URI | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.jbsoc.or.jp/event/journal.html#JB | |||||||||||
