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3-D collagen-dependent cell surface expression of MT1-MMP and MMP-2 activation regardless of integrin β1 function and matrix stiffness
https://doi.org/10.24517/00027410
https://doi.org/10.24517/000274104ad07a32-b885-402d-861a-eb5346f42180
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CA-PR-SAKAI-K-98.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2017-10-05 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 3-D collagen-dependent cell surface expression of MT1-MMP and MMP-2 activation regardless of integrin β1 function and matrix stiffness | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.24517/00027410 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
Sakai, Katsuya
× Sakai, Katsuya× Nakamura, Takahiro× Suzuki, Yoshinori× Imizu, Takafumi× 松本, 邦夫 |
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| 著者別表示 |
酒井, 克也
× 酒井, 克也
× 中村, 隆弘
× 松本, 邦夫
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| 提供者所属 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | 金沢大学がん進展制御研究所 | |||||||||||
| 書誌情報 |
Biochemical and Biophysical Research Communications 巻 412, 号 1, p. 98-103, 発行日 2011-08-01 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0006-291X | |||||||||||
| NCID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00564395 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1016/j.bbrc.2011.07.050 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Elsevier | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Matrix metalloproteinases (MMPs) play roles in spatially dynamic processes, including morphogenesis, wound healing, and tumor invasion. Three-dimensional (3-D) type I collagen stimulates cellular activation of MMP-2, however, the mechanisms underlying this are controversial. The present study investigated mechanisms for 3-D collagen-induced MMP-2 activation in highly invasive human malignant mesothelioma cells. MMP-2 was effectively activated by cells cultured in 3-D collagen but not in 2-D collagen, whereas MMP-2 activation was not regulated by the flexibility of collagen. The 3-D collagen did not largely increase the gene expression of MMP-2 and MT1-MMP. However, MT1-MMP exposed to the cell surface was much increased by 3-D collagen, and loss of MT1-MMP abolished MMP-2 activation in response to 3-D collagen. MT1-MMP and integrin β1 translocated to pericellular regions interacting with collagen-coated microbeads, however their localization was different. Importantly, inhibition of integrin β1 function and expression did not affect 3-D collagen-induced cell surface localization of MT1-MMP and MMP-2 activation. Our results strongly suggest that 3-D collagen scaffolding may provide opportunity for direct and multivalent interaction with MT1-MMP, by which MMP-2 activation occur in abundant cell surface MT1-MMP-dependent manner, rather than a manner regulated by matrix stiffness and integrin β1 function. © 2011 Elsevier Inc. All rights reserved. | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||
| 関連URI | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.elsevier.com/locate/issn/0006291X | |||||||||||
