| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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|
タイトル |
3-D collagen-dependent cell surface expression of MT1-MMP and MMP-2 activation regardless of integrin β1 function and matrix stiffness |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00027410 |
|
ID登録タイプ |
JaLC |
| 著者 |
Sakai, Katsuya
Nakamura, Takahiro
Suzuki, Yoshinori
Imizu, Takafumi
松本, 邦夫
|
| 著者別表示 |
酒井, 克也
中村, 隆弘
松本, 邦夫
|
| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学がん進展制御研究所 |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 412,
号 1,
p. 98-103,
発行日 2011-08-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-291X |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564395 |
| DOI |
|
|
関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.bbrc.2011.07.050 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Matrix metalloproteinases (MMPs) play roles in spatially dynamic processes, including morphogenesis, wound healing, and tumor invasion. Three-dimensional (3-D) type I collagen stimulates cellular activation of MMP-2, however, the mechanisms underlying this are controversial. The present study investigated mechanisms for 3-D collagen-induced MMP-2 activation in highly invasive human malignant mesothelioma cells. MMP-2 was effectively activated by cells cultured in 3-D collagen but not in 2-D collagen, whereas MMP-2 activation was not regulated by the flexibility of collagen. The 3-D collagen did not largely increase the gene expression of MMP-2 and MT1-MMP. However, MT1-MMP exposed to the cell surface was much increased by 3-D collagen, and loss of MT1-MMP abolished MMP-2 activation in response to 3-D collagen. MT1-MMP and integrin β1 translocated to pericellular regions interacting with collagen-coated microbeads, however their localization was different. Importantly, inhibition of integrin β1 function and expression did not affect 3-D collagen-induced cell surface localization of MT1-MMP and MMP-2 activation. Our results strongly suggest that 3-D collagen scaffolding may provide opportunity for direct and multivalent interaction with MT1-MMP, by which MMP-2 activation occur in abundant cell surface MT1-MMP-dependent manner, rather than a manner regulated by matrix stiffness and integrin β1 function. © 2011 Elsevier Inc. All rights reserved. |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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識別子タイプ |
URI |
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|
関連識別子 |
http://www.elsevier.com/locate/issn/0006291X |
CA-PR-SAKAI-K-98.pdf (1.3 MB)
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