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Human cytomegalovirus persistent infection in a human central nervous system cell line: Production of a variant virus with different growth characteristics
http://hdl.handle.net/2297/29184
http://hdl.handle.net/2297/29184e87f9ce6-48cd-48a2-b3e1-cb1145abb405
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CA-PR-SATO-H-2605.pdf (5.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-05 | |||||
| タイトル | ||||||
| タイトル | Human cytomegalovirus persistent infection in a human central nervous system cell line: Production of a variant virus with different growth characteristics | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ogura, Tsutomu
× Ogura, Tsutomu× Tanaka, Junji× Kamiya, Shigeru× Sato, Hiroshi× Ogura, Hisashi× Hatano, Motoichi |
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| 書誌情報 |
Journal of General Virology 巻 67, 号 12, p. 2605-2616, 発行日 1986-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-1317 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00698722 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1099/0022-1317-67-12-2605 | |||||
| 出版者 | ||||||
| 出版者 | Society for General Microbiology | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The susceptibility of human central nervous system cell lines to human cytomegalovirus (HCMV) and the fate of infected cultures were studied. Significant amounts of infectious progeny virus were produced in 118MGC glioma and IMR-32 neuroblastoma, but not in KGC oligodendroglioma cells when the cultures were infected with wild-type virus (HCMVwt) at an m.o.i. of 10 p.f.u. per cell. Further passage of infected 118MGC cells resulted in the establishment of a long-term persistent infection. This infection, designated 118MGC/Towne, continuously produced infectious virus (HCMVpi) with titres ranging from 102 to 105 p.f.u./106 cells up to 360 days post-infection (corresponding to 50 subcultures). Since no temperature-sensitive mutants, defective interfering particles or interferon-like activity were found in the 118MGC/Towne cultures, maintenance of the persistent infection seemed to be due to a balance between the release of infectious virus and the growth of uninfected cells. The HCMVpi produced in long-term persistently infected cultures was shown to be different from the HCMVwt originally used to infect by the following characteristics: (i) HCMVpi replicated slowly and yielded lower amounts of progeny virus than HCMVwt; (ii) HCMVpi induced a 73000 mol. wt. immediate early protein that was not synthesized in HCMVwt-infected cells; (iii) HCMVpi had a different DNA structure from that of HCMVwt. These results suggest that HCMVpi is a slower growing variant of HCMVwt and probably plays an important role in the maintenance of the persistent infection. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
