| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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タイトル |
Caspase-1 protein induces apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)-mediated necrosis independently of its catalytic activity |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00027423 |
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ID登録タイプ |
JaLC |
| 著者 |
Motani, Kou
Kushiyama, Hiroko
Imamura, Ryu
Kinoshita, Takeshi
Nishiuchi, Takumi
Suda, Takashi
|
| 著者別表示 |
今村, 龍
木下, 健
西内, 巧
須田, 貴司
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| 書誌情報 |
Journal of Biological Chemistry
巻 286,
号 39,
p. 33963-33972,
発行日 2011-10-30
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-9258 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00251083 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1074/jbc.M111.286823 |
| 出版者 |
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出版者 |
American Society for Biochemistry and Molecular Biology |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The adaptor protein, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), connects pathogen/danger sensors such as NLRP3 and NLRC4 with caspases and is involved in inflammation and cell death. We have found that ASC activation induced caspase-8-dependent apoptosis or CA-074Me (cathepsin B inhibitor)-inhibitable necrosis depending on the cell type. Unlike necroptosis, another necrotic cell death, ASC-mediated necrosis, was neither RIP3-dependent nor necrostatin-1-inhibitable. Although acetyl-YVAD- chloromethylketone (Ac-YVAD-CMK) (caspase-1 inhibitor) did not inhibit ASC-mediated necrosis, comprehensive gene expression analyses indicated that caspase-1 expression coincided with the necrosis type. Furthermore, caspase-1 knockdown converted necrosis-type cells to apoptosis-type cells, whereas exogenous expression of either wild-type or catalytically inactive caspase-1 did the opposite. Knockdown of caspase-1, but not Ac-YVAD-CMK, suppressed the monocyte necrosis induced by Staphylococcus and Pseudomonas infection. Thus, the catalytic activity of caspase-1 is dispensable for necrosis induction. Intriguingly, a short period of caspase-1 knockdown inhibited IL-1β production but not necrosis, although longer knockdown suppressed both responses. Possible explanations of this phenomenon are discussed. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. |
| 権利 |
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権利情報 |
2012-10-30 |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
CA-PR-SUDA-T-33963.pdf (1.1 MB)
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