| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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|
タイトル |
Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00027454 |
|
ID登録タイプ |
JaLC |
| 著者 |
Ju, Xiaoli
Ishikawa, Tomo-o
Naka, Kazuhito
Ito, Kosei
Ito, Yoshiaki
Oshima, Masanobu
|
| 著者別表示 |
石川, 智夫
仲, 一仁
大島, 正伸
|
| 書誌情報 |
Cancer Science
巻 105,
号 4,
p. 418-424,
発行日 2014-04-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1347-9032 |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11808050 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1111/cas.12356 |
| 出版者 |
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出版者 |
Japanese Cancer Association / Wiley Open Access |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
RUNX3 is a tumor suppressor for a variety of cancers. RUNX3 suppresses the canonical Wnt signaling pathway by binding to the TCF4/β-catenin complex, resulting in the inhibition of binding of the complex to the Wnt target gene promoter. Here, we confirmed that RUNX3 suppressed Wnt signaling activity in several gastric cancer cell lines; however, we found that RUNX3 increased the Wnt signaling activity in KatoIII and SNU668 gastric cancer cells. Notably, RUNX3 expression increased the ratio of the Wnt signaling-high population in the KatoIII cells. although the maximum Wnt activation level of individual cells was similar to that in the control. As found previously, RUNX3 also binds to TCF4 and β-catenin in KatoIII cells, suggesting that these molecules form a ternary complex. Moreover, the ChIP analyses revealed that TCF4, β-catenin and RUNX3 bind the promoter region of the Wnt target genes, Axin2 and c-Myc, and the occupancy of TCF4 and β-catenin in these promoter regions is increased by the RUNX3 expression. These results suggest that RUNX3 stabilizes the TCF4/β-catenin complex on the Wnt target gene promoter in KatoIII cells, leading to activation of Wnt signaling. Although RUNX3 increased the Wnt signaling activity, its expression resulted in suppression of tumorigenesis of KatoIII cells, indicating that RUNX3 plays a tumor-suppressing role in KatoIII cells through a Wnt-independent mechanism. These results indicate that RUNX3 can either suppress or activate the Wnt signaling pathway through its binding to the TCF4/β-catenin complex by cell context-dependent mechanisms. © 2014 The Authors. |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
CA-PR-OSHIMA-M-418.pdf (1.9 MB)
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