| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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|
タイトル |
Substrate choice of membrane-type 1 matrix metalloproteinase is dictated by tissue inhibitor of metalloproteinase-2 levels |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.24517/00027543 |
|
ID登録タイプ |
JaLC |
| 著者 |
Kudo, Tomoya
Takino, Takahisa
Miyamori, Hisashi
Thompson, Erik W.
Sato, Hiroshi
|
| 著者別表示 |
滝野, 隆久
宮森, 久志
佐藤, 博
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| 提供者所属 |
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内容記述タイプ |
Other |
|
内容記述 |
金沢大学がん研究所がん病態制御 |
| 書誌情報 |
Cancer Science
巻 98,
号 4,
p. 563-568,
発行日 2007-04-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1347-9032 |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11808050 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1111/j.1349-7006.2007.00426.x |
| 出版者 |
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出版者 |
Japanese Cancer Association / Oxford University Press |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Although tissue inhibitor of metalloproteinase-2 (TIMP-2) is known to be not only an inhibitor of matrix metalloproteinases (MMP) but also a cofactor for membrane-type 1 MMP (MT1-MMP)-mediated MMP-2 activation, it is still unclear how TIMP-2 regulates MMP-2 activation and cleavage of substrates by MT1-MMP. In the present study we examined the levels of cell-surface MT1-MMP, MMP-2 activation and cleavage of MT1-MMP substrates in 293T cells transfected with the MT1-MMP and TIMP-2 genes. Co-expression of TIMP-2 at an appropriate level increased the level of cell-surface MT1-MMP, both the TIMP-2-bound and free forms, and generated processed MMP-2 with gelatin-degrading activity. In contrast, MT1-MMP substrates testican-1 and syndecan-1 were cleaved by the cells expressing MT1-MMP, which was inhibited by TIMP-2 even at levels that stimulate MMP-2 activation. These results suggest that TIMP-2 environment determines MT1-MMP substrate choice between direct cleavage of its own substrates and MMP-2 activation. © 2007 Japanese Cancer Association. |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
CA-PR-SATO-H-563.pdf (542.4 kB)
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