| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-05 |
| タイトル |
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タイトル |
Dopamine release via the vacuolar ATPase V0 sector c-subunit, confirmed in N18 neuroblastoma cells, results in behavioral recovery in hemiparkinsonian mice |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00030985 |
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ID登録タイプ |
JaLC |
| 著者 |
Duo, Jin
Muramatsu, Shin-ichi
Shimizu, Nobuaki
Yokoyama, Shigeru
Hirai, Hirokazu
Yamada, Kiyofumi
Liu, Hong-Xiang
Higashida, Chiharu
Hashii, Minako
Higashida, Akihiko
Asano, Masahide
Ohkuma, Shoji
Higashida, Haruhiro
|
| 著者別表示 |
清水, 宣明
横山, 茂
山田, 清文
橋井, 美奈子
浅野, 雅秀
大熊, 勝治
東田, 陽博
|
| 書誌情報 |
Neurochemistry International
巻 61,
号 6,
p. 907-912,
発行日 2012-11-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0197-0186 |
| NCID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA0032399X |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.neuint.2011.12.021 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
A 16-kDa proteolipid, mediatophore, in Torpedo electric organs mediates Ca 2+-dependent acetylcholine release. Mediatophore is identical to the pore-forming stalk c-subunit of the V0 sector of vacuolar proton ATPase (ATP6V0C). The function of ATP6V0C in the mammalian central nervous system is not clear. Here, we report transfection of adeno-associated viral vectors harboring rat ATP6V0C into the mouse substantia nigra, in which high potassium stimulation increased overflow of endogenous dopamine (DA) measured in the striatum by in vivo microdialysis. Next, in the striatum of 6-hydroxydopamine- lesioned mice, a model of Parkinson's disease (PD), human tyrosine hydroxylase, aromatic l-amino-acid decarboxylase and guanosine triphosphate cyclohydrolase 1, together with or without ATP6V0C, were expressed in the caudoputamen for rescue. Motor performance on the accelerating rotarod test and amphetamine-induced ipsilateral rotation were improved in the rescued mice coexpressing ATP6V0C. [ 3H]DA, taken up into cultured N18 neuronal tumor cells transformed to express ATP6V0C, was released by potassium stimulation. These results indicated that ATP6V0C mediates DA release from nerve terminals in the striatum of DA neurons of normal mice and from gene-transferred striatal cells of parkinsonian mice. The results suggested that ATP6V0C may be useful as a rescue molecule in addition to DA-synthetic enzymes in the gene therapy of PD. © 2011 Elsevier Ltd. All rights reserved. |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連URI |
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識別子タイプ |
URI |
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関連識別子 |
http://www.elsevier.com/locate/issn/01970186 |
ME-PR-HIGASHIDA-H-907.pdf (187.9 kB)
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