| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-22 |
| タイトル |
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タイトル |
Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: Application to behavioral and developmental studies |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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ID登録 |
10.24517/00049522 |
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ID登録タイプ |
JaLC |
| 著者 |
Hara, Chiho
Morishita, Koudai
Takayanagi-Kiya, Seika
Mikami, Akihisa
Uchino, Keiro
Sakurai, Takeshi
Kanzaki, Ryohei
Sezutsu, Hideki
Iwami, Masafumi
Kiya, Taketoshi
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| 著者別表示 |
木矢, 星歌
櫻井, 武
岩見, 雅史
木矢, 剛智
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| 提供者所属 |
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内容記述タイプ |
Other |
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内容記述 |
金沢大学理工研究域自然システム学系 |
| 書誌情報 |
Scientific Reports
巻 7,
号 1,
p. 11795,
発行日 2017-12-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
2045-2322 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41598-017-12102-2 |
| 出版者 |
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出版者 |
Nature Publishing Group |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Silkmoth, Bombyx mori, is one of the important model insects in which transgenic techniques and the GAL4/UAS system are applicable. However, due to cytotoxicity and low transactivation activity of GAL4, effectiveness of the GAL4/UAS system and its application in B. mori are still limited. In the present study, we refined the previously reported UAS vector by exploiting transcriptional and translational enhancers, and achieved 200-fold enhancement of reporter GFP fluorescence in the GAL4/UAS system. Enhanced protein expression of membrane-targeted GFP and calcium indicator protein (GCaMP5G) drastically improved visualization of fine neurite structures and neural activity, respectively. Also, with the refined system, we generated a transgenic strain that expresses tetanus toxin light chain (TeTxLC), which blocks synaptic transmission, under the control of GAL4. Ectopic TeTxLC expression in the sex pheromone receptor neurons inhibited male courtship behavior, proving effectiveness of TeTxLC on loss-of-function analyses of neural circuits. In addition, suppression of prothoracicotropic hormone (PTTH) or insulin-like peptide (bombyxin) secretion impaired developmental timing and growth rate, respectively. Furthermore, we revealed that larval growth is sex-differentially regulated by these peptide hormones. The present study provides important technical underpinnings of transgenic approaches in silkmoths and insights into mechanisms of postembryonic development in insects. © 2017 The Author(s). |
| 権利 |
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権利情報 |
Copyright © 2017 The Author(s). |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
SC-PR-KIYA-T-11795.pdf (8.1 MB)
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