| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-25 |
| タイトル |
|
|
タイトル |
Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00049602 |
|
ID登録タイプ |
JaLC |
| 著者 |
Kagami, Kyosuke
Shinmyo, Yohei
Ono, Masanori
Kawasaki, Hiroshi
Fujiwara, Hiroshi
|
| 著者別表示 |
鏡, 京介
新明, 洋平
河﨑, 洋志
藤原, 浩
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学医薬保健研究域医学系 |
| 書誌情報 |
Scientific Reports
巻 7,
号 1,
p. 5964,
発行日 2017-12-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2045-2322 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41598-017-06549-6 |
| 出版者 |
|
|
出版者 |
Nature Publishing Group |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placenta through the uterus using a modified tissue clearing CUBIC method. By this procedure, we can recognize the three-dimensional relationships among various tissues within the pregnant uterus and analyze free-angle images of cross-sections with single-cell resolution using a computer system. Based on these data, we can select optimal cross-section angles and then produce the corresponding tissue slices that are adequate for further immunohistochemical examination. Furthermore, using transgenic mice, distinct images of an EGFP-positive embryo and the placenta can be obtained, confirming the precise three-dimensional location of invading trophoblasts in the feto-maternal interface in the uterus. These results indicate that this procedure will significantly contribute to analyzing pathophysiological mechanisms in reproductive organs. © 2017 The Author(s). |
| 権利 |
|
|
権利情報 |
Copyright © 2017 The Author(s). |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ME-PR-KAWASAKI-H-5964.pdf (3.2 MB)
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