| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-12-28 |
| タイトル |
|
|
タイトル |
Real-space and real-Time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00049632 |
|
ID登録タイプ |
JaLC |
| 著者 |
Shibata, Mikihiro
Nishimasu, Hiroshi
Kodera, Noriyuki
Hirano, Seiichi
Ando, Toshio
Uchihashi, Takayuki
Nureki, Osamu
|
| 著者別表示 |
柴田, 幹大
古寺, 哲幸
安藤, 敏夫
内橋, 貴之
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
金沢大学ナノ生命科学研究所 |
| 書誌情報 |
Nature Communications
巻 8,
号 1,
p. 1430,
発行日 2017-12-01
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2041-1723 |
| NCID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12645905 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1038/s41467-017-01466-8 |
| 出版者 |
|
|
出版者 |
Nature Publishing Group |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
The CRISPR-Associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9-RNA system has been harnessed for numerous applications, such as genome editing. Here we use high-speed atomic force microscopy (HS-AFM) to visualize the real-space and real-Time dynamics of CRISPR-Cas9 in action. HS-AFM movies indicate that, whereas apo-Cas9 adopts unexpected flexible conformations, Cas9-RNA forms a stable bilobed structure and interrogates target sites on the DNA by three-dimensional diffusion. These movies also provide real-Time visualization of the Cas9-mediated DNA cleavage process. Notably, the Cas9 HNH nuclease domain fluctuates upon DNA binding, and subsequently adopts an active conformation, where the HNH active site is docked at the cleavage site in the target DNA. Collectively, our HS-AFM data extend our understanding of the action mechanism of CRISPR-Cas9. © 2017 The Author(s). |
| 内容記述 |
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|
内容記述タイプ |
Other |
|
内容記述 |
出版社版 |
| 権利 |
|
|
権利情報 |
Copyright © Nature Publishing Group |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
FR-PR-SHIBATA-M-1430.pdf (4.5 MB)
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